Product: CD44 Antibody
Catalog: AF6186
Description: Rabbit polyclonal antibody to CD44
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90kDa; 82kD(Calculated).
Uniprot: P16070
RRID: AB_2835069

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(89%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CD44 Antibody detects endogenous levels of total CD44.
RRID:
AB_2835069
Cite Format: Affinity Biosciences Cat# AF6186, RRID:AB_2835069.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LHR; BA-1; CD 44; CD44; CD44 antigen; CD44 molecule (Indian blood group); CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; IN; INLU-related p80 Glycoprotein; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH1; PGP-1; PGP-I; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; Soluble CD44;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P16070 CD44_HUMAN:

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Description:
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs).
Sequence:
MDKFWWHAAWGLCLVPLSLAQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPKEDSHSTTGTAAASAHTSHPMQGRTTPSPEDSSWTDFFNPISHPMGRGHQAGRRMDMDSSHSITLQPTANPNTGLVEDLDRTGPLSMTTQQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPHTKESRTFIPVTSAKTGSFGVTAVTVGDSNSNVNRSLSGDQDTFHPSGGSHTTHGSESDGHSHGSQEGGANTTSGPIRTPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINSGNGAVEDRKPSGLNGEASKSQEMVHLVNKESSETPDQFMTADETRNLQNVDMKIGV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Horse
89
Chicken
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16070 As Substrate

Site PTM Type Enzyme
S45 Phosphorylation
K54 Ubiquitination
N57 N-Glycosylation
S58 Phosphorylation
T62 Phosphorylation
N110 N-Glycosylation
K158 Ubiquitination
T163 Phosphorylation
S171 Phosphorylation
T174 O-Glycosylation
S179 Phosphorylation
S182 Phosphorylation
S183 Phosphorylation
S184 Phosphorylation
T197 O-Glycosylation
S199 O-Glycosylation
T200 O-Glycosylation
T222 Phosphorylation
S291 Phosphorylation
T311 Phosphorylation
T318 O-Glycosylation
T325 O-Glycosylation
S330 O-Glycosylation
S332 O-Glycosylation
Y412 Phosphorylation
S420 Phosphorylation
S422 O-Glycosylation
T423 O-Glycosylation
T424 O-Glycosylation
T426 O-Glycosylation
S430 O-Glycosylation
T433 O-Glycosylation
S434 O-Glycosylation
S458 O-Glycosylation
S514 O-Glycosylation
T515 O-Glycosylation
T528 O-Glycosylation
T529 O-Glycosylation
T541 O-Glycosylation
S550 O-Glycosylation
S553 O-Glycosylation
T554 O-Glycosylation
T555 O-Glycosylation
T561 O-Glycosylation
S562 O-Glycosylation
T567 O-Glycosylation
S570 O-Glycosylation
T572 O-Glycosylation
T572 Phosphorylation
T577 O-Glycosylation
S578 O-Glycosylation
T581 O-Glycosylation
S583 O-Glycosylation
T587 Phosphorylation
S672 Phosphorylation P17252 (PRKCA)
K681 Ubiquitination
S686 Phosphorylation
K695 Ubiquitination
S697 Phosphorylation P17612 (PRKACA)
S704 Phosphorylation
K705 Ubiquitination
S706 Phosphorylation Q9UQM7 (CAMK2A)
K715 Ubiquitination
S717 Phosphorylation
S718 Phosphorylation
T720 Phosphorylation
T726 Phosphorylation
K739 Ubiquitination

Research Backgrounds

Function:

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.

PTMs:

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.

N-glycosylated.

O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.

Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Microvillus.
Note: Colocalizes with actin in membrane protrusions at wounding edges. Co-localizes with RDX, EZR and MSN in microvilli. Localizes to cholesterol-rich membrane-bound lipid raft domains.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Subunit Structure:

Interacts with PKN2. Interacts with TIAM1 and TIAM2 (By similarity). Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with UNC119. Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration. Interacts with RDX, EZR and MSN (By similarity). Interacts with EGFR. Interacts with CD74; this complex is essential for the MIF-induced signaling cascade that results in B cell survival (By similarity).

Family&Domains:

The lectin-like LINK domain is responsible for hyaluronan binding.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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