HAT Antibody | Affinity Biosciences

Product:	HAT Antibody
Catalog:	AF0269
Description:	Rabbit polyclonal antibody to HAT
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	49kDa; 50kD(Calculated).
Uniprot:	O14929
RRID:	AB_2833443

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

HAT Antibody detects endogenous levels of total HAT.

RRID:

AB_2833443
Cite Format: Affinity Biosciences Cat# AF0269, RRID:AB_2833443.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HAT 1; hat1; HAT1_HUMAN; Histidine aminotransferase 1; Histone acetyltransferase 1; Histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; KAT1;

Immunogens

Immunogen:

Uniprot:

O14929(HAT1_HUMAN) >>Visit HPA database.

Gene(ID):

HAT1(8520)

Description:

HAT1 May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at 'Lys-5' and 'Lys-12' and acetylates histone H2A at 'Lys-5'. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Belongs to the HAT1 family.

Sequence:

O14929 HAT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGFGAMEKFLVEYKSAVEKKLAEYKCNTNTAIELKLVRFPEDLENDIRTFFPEYTHQLFGDDETAFGYKGLKILLYYIAGSLSTMFRVEYASKVDENFDCVEADDVEGKIRQIIPPGFCTNTNDFLSLLEKEVDFKPFGTLLHTYSVLSPTGGENFTFQIYKADMTCRGFREYHERLQTFLMWFIETASFIDVDDERWHYFLVFEKYNKDGATLFATVGYMTVYNYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSKSYVKLRDFVLVKLCQDLPCFSREKLMQGFNEDMAIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLAQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14929 As Substrate

O14929

Site	PTM Type	Source
A2	Acetylation	Uniprot
K9	Ubiquitination	Uniprot
K15	Acetylation	Uniprot
K15	Ubiquitination	Uniprot
K26	Ubiquitination	Uniprot
K36	Ubiquitination	Uniprot
T50	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
Y69	Phosphorylation	Uniprot
K70	Ubiquitination	Uniprot
Y78	Phosphorylation	Uniprot
S93	Phosphorylation	Uniprot
C101	S-Nitrosylation	Uniprot
K110	Ubiquitination	Uniprot
S190	Phosphorylation	Uniprot
T245	Phosphorylation	Uniprot
K280	Acetylation	Uniprot
K280	Ubiquitination	Uniprot
R286	Methylation	Uniprot
K304	Ubiquitination	Uniprot
K320	Ubiquitination	Uniprot
S343	Phosphorylation	Uniprot
S350	Phosphorylation	Uniprot
Y351	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
Y363	Phosphorylation	Uniprot

Research Backgrounds

O14929_HAT1_HUMAN

Function:

Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.

Subcellular Location:

Nucleus matrix.

Cytoplasm. Nucleus. Nucleus matrix. Nucleus>Nucleoplasm.
Note: Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.

Subunit Structure:

Catalytic subunit of the type B histone acetyltransferase (HAT) complex, composed of RBBP7 and HAT1. Interacts with histones H4 and H2A. The interaction is dependent of the ability of RBBP7 to bind to the N-terminus of histones. Component of the histone H3.1 and H3.3 complexes.

Family&Domains:

Belongs to the HAT1 family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

References

1). A novel lncRNA SNHG29 regulates EP300- related histone acetylation modification and inhibits FLT3-ITD AML development. Leukemia, 2023 (PubMed: 37157016) [IF=12.8]

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HAT Antibody - #AF0269