TNNI3 Antibody - #AF6214
*The optimal dilutions should be determined by the end user.
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6214, RRID:AB_2835095.
cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P19429 As Substrate
|S23||Phosphorylation||Q13976 (PRKG1) , Q15139 (PRKD1) , P17612 (PRKACA) , Q05655 (PRKCD) , Q13131 (PRKAA1) , P17252 (PRKCA)||Uniprot|
|S24||Phosphorylation||Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q13131 (PRKAA1)||Uniprot|
|S42||Phosphorylation||P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)||Uniprot|
|S44||Phosphorylation||P05771 (PRKCB) , P17252 (PRKCA) , Q02156 (PRKCE) , P05129 (PRKCG)||Uniprot|
|T143||Phosphorylation||Q05655 (PRKCD) , Q13043 (STK4) , P05771 (PRKCB) , P17252 (PRKCA)||Uniprot|
|S150||Phosphorylation||O75914 (PAK3) , Q13131 (PRKAA1)||Uniprot|
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1.
Belongs to the troponin I family.
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.