Product: MKP1/2 Antibody
Catalog: AF6243
Description: Rabbit polyclonal antibody to MKP1/2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 41kDa; 39kD,43kD(Calculated).
Uniprot: P28562 | Q13115
RRID: AB_2835107

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MKP1/2 Antibody detects endogenous levels of total MKP1/2.
RRID:
AB_2835107
Cite Format: Affinity Biosciences Cat# AF6243, RRID:AB_2835107.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; Dual specificity phosphatase 4; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; DUS4_HUMAN; DUSP 4; Dusp4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; Mitogen-activated protein kinase phosphatase 2; MKP 2; MKP-2; MKP2; Serine/threonine specific protein phosphatase; TYP; Typ1; VH 2; VH1 homologous phosphatase 2; VH2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P28562 DUS1_HUMAN:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

Description:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.
Sequence:
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC

MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28562/Q13115 As Substrate

Site PTM Type Enzyme
K57 Acetylation PR:000007102 (EP300)
K57 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K122 Ubiquitination
S131 Phosphorylation
S140 Phosphorylation
T141 Phosphorylation
S146 Phosphorylation
K221 Ubiquitination
K230 Ubiquitination
K280 Ubiquitination
K289 Ubiquitination
S296 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S323 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S334 Phosphorylation P54646 (PRKAA2)
S359 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S364 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
Site PTM Type Enzyme
V2 Acetylation
K16 Ubiquitination
Y60 Phosphorylation
S64 Phosphorylation
T71 Phosphorylation
K78 Ubiquitination
Y102 Phosphorylation
Y108 Phosphorylation
S112 Phosphorylation
Y154 Phosphorylation
S159 Phosphorylation
K160 Ubiquitination
K311 Ubiquitination
S345 Phosphorylation
T356 Phosphorylation
T359 Phosphorylation
S386 Phosphorylation
S391 Phosphorylation

Research Backgrounds

Function:

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

PTMs:

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Function:

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

PTMs:

Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). Fan C et al. Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice. Chinese Journal of Natural Medicines 2023 Jun;21(411-422) (PubMed: 37407172) [IF=4.6]

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