VHL Antibody - #AF6292
(2)
(2)
| Product: | VHL Antibody |
| Catalog: | AF6292 |
| Description: | Rabbit polyclonal antibody to VHL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Dog |
| Mol.Wt.: | 25kDa; 24kD(Calculated). |
| Uniprot: | P40337 |
| RRID: | AB_2835142 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6292, RRID:AB_2835142.
Fold/Unfold
Elongin binding protein; G7 protein; HRCA 1; HRCA1; Protein G7; pVHL; RCA 1; RCA1; VHL 1; VHL; VHL_HUMAN; VHL1; VHLH; Von Hippel Lindau disease tumor suppressor; von Hippel Lindau syndrome; von Hippel Lindau tumor suppressor; Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase; Von Hippel-Lindau disease tumor suppressor;
Immunogens
- P40337 VHL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P40337 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S33 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S38 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S43 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S68 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S72 | Phosphorylation | O14965 (AURKA) , P48729 (CSNK1A1) | Uniprot |
| R79 | Methylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| T100 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| T105 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| S111 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| Y112 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| K159 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| K171 | Sumoylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| Y175 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| S183 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
| Y185 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T202 | Phosphorylation | Q96PY6 (NEK1) | Uniprot |
Research Backgrounds
Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.
Cytoplasm. Membrane>Peripheral membrane protein. Nucleus.
Note: Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated. Colocalizes with ADRB2 at the cell membrane.
Cytoplasm. Nucleus.
Note: Equally distributed between the nucleus and the cytoplasm but not membrane-associated.
Expressed in the adult and fetal brain and kidney.
Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins. Interacts with CUL2; this interaction is dependent on the integrity of the trimeric VBC complex. Interacts (via the beta domain) with HIF1A (via the NTAD domain); this interaction mediates degradation of HIF1A in normoxia and, in hypoxia, prevents ubiquitination and degradation of HIF1A by mediating hypoxia-induced translocation to the nucleus, a process which requires a hypoxia-dependent regulatory signal. Interacts with ADRB2; the interaction, in normoxia, is dependent on hydroxylation of ADRB2 and the subsequent VCB-mediated ubiquitination and degradation of ADRB2. Under hypoxia, hydroxylation, interaction with VHL, ubiquitination and subsequent degradation of ADRB2 are dramatically decreased. Interacts with RNF139, USP33 and JADE1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Isoform 1 and isoform 3 interact with LIMD1 (via LIM zinc-binding 2), AJUBA (via LIM domains) and WTIP (via LIM domains). Interacts with EPAS1. Interacts with CARD9.
The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Belongs to the VHL family.
Research Fields
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
References
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