Product: G3BP-1 Antibody
Catalog: AF6427
Description: Rabbit polyclonal antibody to G3BP-1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 60kDa; 52kD(Calculated).
Uniprot: Q13283
RRID: AB_2835160

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Dog(90%)
Clonality:
Polyclonal
Specificity:
G3BP-1 Antibody detects endogenous levels of total G3BP-1.
RRID:
AB_2835160
Cite Format: Affinity Biosciences Cat# AF6427, RRID:AB_2835160.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent DNA helicase VIII; ATP-dependent DNA helicase VIII; G3BP; G3BP stress granule assembly factor 1; G3BP-1; G3bp1; G3BP1_HUMAN; GAP binding protein; GAP SH3 domain binding protein 1; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; Human DNA helicase VIII; MGC111040; Ras GTPase activating protein binding protein 1; Ras GTPase activating protein SH3 domain binding protein; Ras GTPase-activating protein-binding protein 1; RasGAP associated endoribonuclease G3BP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13283 G3BP1_HUMAN:

Ubiquitous.

Description:
G3BP-1 an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs.
Sequence:
MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
91
Bovine
91
Sheep
91
Dog
90
Xenopus
67
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13283 As Substrate

Site PTM Type Enzyme
K5 Methylation
K5 Ubiquitination
S7 Phosphorylation
Y20 Phosphorylation
K36 Ubiquitination
S47 Phosphorylation
K50 Ubiquitination
Y56 Phosphorylation P06239 (LCK)
K59 Ubiquitination
K64 Ubiquitination
S67 Phosphorylation
T71 Phosphorylation
T75 Phosphorylation
K76 Acetylation
K76 Ubiquitination
S119 Phosphorylation
K123 Ubiquitination
Y125 Phosphorylation
Y133 Phosphorylation P12931 (SRC)
T143 Phosphorylation
S149 Phosphorylation
T162 Phosphorylation
T222 Phosphorylation
S230 Phosphorylation
S231 Phosphorylation
S232 Phosphorylation
T241 Phosphorylation
R247 Methylation
S250 Phosphorylation
S253 Phosphorylation
K287 Ubiquitination
S339 Phosphorylation
K353 Ubiquitination
K357 Ubiquitination
S362 Phosphorylation
Y363 Phosphorylation
S373 Phosphorylation
K376 Acetylation
K376 Ubiquitination
S388 Phosphorylation
K393 Ubiquitination
R403 Methylation
R427 Methylation
R429 Methylation
R435 Methylation
R443 Methylation
R447 Methylation
K453 Methylation
R460 Methylation
R465 Methylation

Research Backgrounds

Function:

ATP- and magnesium-dependent helicase that plays an essential role in innate immunity. Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA. In addition, plays an essential role in stress granule formation. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR.

PTMs:

(Microbial infection) Cleaved by human enterovirus 71; this cleavage induces the disassembly of cytoplasmic stress granules. Cleaved by Foot-and-mouth disease virus; this cleavage suppresses the formation of cytoplasmic stress granules.

Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.

Arg-435 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location:

Cytoplasm>Cytosol. Perikaryon. Cytoplasm>Stress granule. Nucleus.
Note: Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer and oligomer. Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP1 (By similarity). Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells (By similarity). No interaction in quiescent cells (By similarity). Interacts (via NTF2-like domain) with USP10. Interacts with RPTOR and SPAG5; this complex is increased by oxidative stress. Interacts with ATXN2L. Interacts with STYXL1. Interacts with CGAS (via N-terminus); this interaction promotes the DNA-binding and activation of CGAS. Interacts (via C-terminus) with DDX58. Interacts (via NTF2-like domain) with CAPRIN1. Interacts with PABPC1.

(Microbial infection) Interacts with Semliki forest virus non-structural protein 3 (via C-terminus); this interaction inhibits the formation of host stress granules on viral mRNAs and the nsp3-G3BP1 complexes bind viral RNAs and probably orchestrate the assembly of viral replication complexes.

(Microbial infection) Interacts with Chikungunya virus non-structural protein 3 (via C-terminus); this interaction inhibits the formation of host stress granules on viral mRNAs and the nsp3-G3BP1 complexes bind viral RNAs and probably orchestrate the assembly of viral replication complexes.

(Microbial infection) Interacts with Sindbis virus non-structural protein 3 (via C-terminus); this interaction inhibits the formation of host stress granules on viral mRNAs and the nsp3-G3BP1 complexes bind viral RNAs and probably orchestrate the assembly of viral replication complexes.

Family&Domains:

The NTF2 domain mediates multimerization.

References

1). Li XY et al. The Role of G3BP1 Gene Mediates P38 MAPK/JNK Pathway in Testicular Spermatogenic Dysfunction Caused by Cyfluthrin. Toxics 2023 May 10;11(5) (PubMed: 37235265) [IF=4.6]

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