Product: HDAC6 Antibody
Catalog: AF6485
Description: Rabbit polyclonal antibody to HDAC6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 131kDa; 131kD(Calculated).
Uniprot: Q9UBN7
RRID: AB_2835165

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

HDAC6 Antibody detects endogenous levels of total HDAC6.
Cite Format: Affinity Biosciences Cat# AF6485, RRID:AB_2835165.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


CPBHM; FLJ16239; HD 6; HD6; HDAC 6; HDAC6; HDAC6_HUMAN; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM 21; JM21; KIAA0901; OTTHUMP00000032398; OTTHUMP00000197663; PPP1R90; Protein phosphatase 1 regulatory subunit 90;


Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.

PTMs - Q9UBN7 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S22 Phosphorylation P49841 (GSK3B)
S28 Phosphorylation
T30 Phosphorylation
K32 Ubiquitination
K36 Acetylation
K37 Acetylation
S43 Phosphorylation
K51 Ubiquitination
K116 Ubiquitination
S412 Phosphorylation
S458 Phosphorylation P68400 (CSNK2A1)
Y570 Phosphorylation P00533 (EGFR)
S846 Phosphorylation
S847 Phosphorylation
K849 Acetylation
K849 Ubiquitination
K853 Acetylation
K854 Acetylation
K854 Ubiquitination
K860 Ubiquitination
T868 Phosphorylation
K873 Acetylation
K881 Acetylation
T923 Phosphorylation
S925 Phosphorylation
T993 Phosphorylation
S995 Phosphorylation
T1003 Phosphorylation
T1016 Phosphorylation
T1021 Phosphorylation
T1027 Phosphorylation
T1031 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
T1034 Phosphorylation
S1035 Phosphorylation P27361 (MAPK3)
T1040 Phosphorylation
T1041 Phosphorylation
T1048 Phosphorylation
T1055 Phosphorylation
S1062 Phosphorylation
K1206 Ubiquitination

Research Backgrounds


Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.


Phosphorylated by AURKA.

Ubiquitinated. Its polyubiquitination however does not lead to its degradation.

Sumoylated in vitro.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Perikaryon. Cell projection>Dendrite. Cell projection>Axon.
Note: It is mainly cytoplasmic, where it is associated with microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SIRT2 (via both phosphorylated, unphosphorylated, active or inactive forms); the interaction is necessary for the complex to interact with alpha-tubulin. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with BBIP10, CBFA2T3, CYLD, DDIT3/CHOP, ZMYND15, F-actin and HDAC11. Interacts with RIPOR2; this interaction occurs during early myogenic differentiation and prevents HDAC6 to deacetylate tubulin. Interacts with DYSF; this interaction occurs during early myogenic differentiation. Interacts with TPPP; inhibiting the tubulin deacetylase activity of HDAC6. Interacts with DYNLL1.


Belongs to the histone deacetylase family. HD type 2 subfamily.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.


1). Inorganic arsenic exposure promotes malignant progression by HDAC6-mediated down-regulation of HTRA1. Journal of Applied Toxicology (PubMed: 36861143) [IF=3.3]

Application: WB    Species: Human    Sample: Caco-2 cells

FIGURE 6 HDAC6 inhibition restored HTRA1 expression in iAs-exposed Caco-2 cells. (A) RT-qPCR result showed the transcript level of HTRA1 in cells from the Ctrl and iAs groups treated with WT-161 (5 μM); DMSO was used as control. (B) The protein levels of HDAC6 and HTRA1 in response to WT-161 treatment were determined by Western blot analysis. (C) Apoptosis of cells treated with panobinostat (100 nM), WT-161 (5 μM), and mocetinostat (1 μM) for 48 h was examined by cytotoxicity assay. The sensitivity of the treatment of WT-161 (5 μM), 5-FU (6 μM) or the combination of these drugs for 48 h was analyzed by (D) cytotoxicity assay and (E) DNA fragmentation ELISA. Data were representatives of at least three independent experiments, shown as mean ± SD. The Significance threshold for one-way ANOVA:

2). Emodin inhibits HDAC6 mediated NLRP3 signaling and relieves chronic inflammatory pain in mice. Experimental and therapeutic medicine (PubMed: 38144917) [IF=2.7]

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