TRADD Antibody | Affinity Biosciences

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Western blot analysis on COS7 and HuvEc cell lysates using TRADD Antibody.

AF0284 at 1/100 staining human brain tissue sections by IHC-P.

Product:	TRADD Antibody
Catalog:	AF0284
Description:	Rabbit polyclonal antibody to TRADD
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	34kDa; 34kD(Calculated).
Uniprot:	Q15628
RRID:	AB_2833452

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%)

Clonality:

Polyclonal

Specificity:

TRADD Antibody detects endogenous levels of total TRADD.

RRID:

AB_2833452
Cite Format: Affinity Biosciences Cat# AF0284, RRID:AB_2833452.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

9130005N23Rik; AA930854; TNFR1 associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A associated via death domain; TNFRSF1A-associated via death domain; tradd; TRADD_HUMAN; Tumor necrosis factor receptor type 1 associated DEATH domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein;

Immunogens

Immunogen:

Uniprot:

Q15628(TRADD_HUMAN) >>Visit HPA database.

Gene(ID):

TRADD(8717)

Expression:

Q15628 TRADD_HUMAN:

Found in all examined tissues.

Description:

TRADD Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

Sequence:

Q15628 TRADD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAGQNGHEEWVGSAYLFVESSLDKVVLSDAYAHPQQKVAVYRALQAALAESGGSPDVLQMLKIHRSDPQLIVQLRFCGRQPCGRFLRAYREGALRAALQRSLAAALAQHSVPLQLELRAGAERLDALLADEERCLSCILAQQPDRLRDEELAELEDALRNLKCGSGARGGDGEVASAPLQPPVPSLSEVKPPPPPPPAQTFLFQGQPVVNRPLSLKDQQTFARSVGLKWRKVGRSLQRGCRALRDPALDSLAYEYEREGLYEQAFQLLRRFVQAEGRRATLQRLVEALEENELTSLAEDLLGLTDPNGGLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Rabbit

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15628 As Substrate

Q15628

Site	PTM Type	Source
K38	Ubiquitination	Uniprot
Y42	Phosphorylation	Uniprot
K163	Ubiquitination	Uniprot
S215	Phosphorylation	Uniprot
K229	Ubiquitination	Uniprot
S296	Phosphorylation	Uniprot

Research Backgrounds

Q15628_TRADD_HUMAN

Function:

The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton.
Note: Shuttles between the cytoplasm and the nucleus.

Tissue Specificity:

Found in all examined tissues.

Subunit Structure:

Stimulation of TNF-alpha receptor TNFRSF1A leads to the formation of two distinct signaling complexes. Plasma membrane-bound complex I is composed of TNFRSF1A, TRADD, RIPK1, TRAF2 and BIRC2/c-IAP1 or BIRC3 which interacts with CHUCK/IKK-alpha, IKBKB/IKK-beta and IKBKG/IKK-gamma promoting cell survival. Subsequently, TRADD, RIPK1 and TRAF2 dissociate from TNFRSF1A and form cytoplasmic complex II with FADD and caspase CASP8 promoting cell apoptosis. Within complex I, interacts with TNFRSF1A/TNFR1, TRAF2 and kinase RIPK1. Within complex I, interacts with TRPC4AP; the interaction promotes NF-kappa B activation. UXT1 associates with complex I; the interaction prevents the formation of complex II. Within complex I Interacts with scaffold protein DAB2IP. Interacts with autophagy receptor SQSTM1. Interacts with E3 ligase TRIP12 (By similarity). Interacts with kinase HIPK2. Interacts with keratin KRT14. Interacts with keratin KRT18. Interacts with keratins KRT16 and KRT17 (By similarity). Interacts with FADD (By similarity).

Family&Domains:

Requires the intact death domain to associate with TNFRSF1A/TNFR1.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). The matrix protein of Newcastle disease virus inhibits inflammatory response through IRAK4/TRAF6/TAK1/NF-κB signaling pathway. International Journal of Biological Macromolecules (PubMed: 35872314) [IF=8.2]

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TRADD Antibody - #AF0284