Product: GSK3 alpha Antibody
Catalog: AF6336
Description: Rabbit polyclonal antibody to GSK3 alpha
Application: WB IHC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: P49840
RRID: AB_2835192

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IP
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%)
GSK3 alpha Antibody detects endogenous levels of total GSK3 alpha.
Cite Format: Affinity Biosciences Cat# AF6336, RRID:AB_2835192.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


DKFZp686D0638; Glycogen synthase kinase 3 alpha; Glycogen synthase kinase-3 alpha; GSK 3 alpha; GSK 3A; GSK-3 alpha; Gsk3a; GSK3A_HUMAN; GSK3alpha; Serine/threonine protein kinase GSK3A;


GSK3A a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49840 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S7 Phosphorylation
S14 Phosphorylation
R16 Methylation
T19 Phosphorylation
S20 Phosphorylation
S21 Phosphorylation Q96BR1 (SGK3) , Q05655 (PRKCD) , Q13554 (CAMK2B) , Q14164 (IKBKE) , P05129 (PRKCG) , P05771 (PRKCB) , P17612 (PRKACA) , P51812 (RPS6KA3) , P24723 (PRKCH) , P31749 (AKT1) , O94806 (PRKD3) , O00141 (SGK1) , P17252 (PRKCA)
S39 Phosphorylation
S41 Phosphorylation
T46 Phosphorylation
S52 Phosphorylation
S63 Phosphorylation
S64 Phosphorylation
S72 Phosphorylation
S77 Phosphorylation
T84 Phosphorylation
S97 Phosphorylation
T101 Phosphorylation
K123 Acetylation
Y134 Phosphorylation
T141 Phosphorylation
K166 Ubiquitination
Y177 Phosphorylation
Y180 Phosphorylation
Y197 Phosphorylation
K246 Ubiquitination
K260 Ubiquitination
S266 Phosphorylation
K268 Ubiquitination
S278 Phosphorylation P49840 (GSK3A)
Y279 Phosphorylation P49840 (GSK3A)
S282 Phosphorylation
Y284 Phosphorylation
Y285 Phosphorylation
T338 Phosphorylation
T340 Phosphorylation
Y351 Phosphorylation
K355 Ubiquitination
K360 Ubiquitination
K366 Ubiquitination
T478 Phosphorylation
T480 Phosphorylation
S482 Phosphorylation

PTMs - P49840 As Enzyme

Substrate Site Source
O15084 (ANKRD28) S1007 Uniprot
O75030-9 (MITF) S298 Uniprot
O75030 (MITF) S405 Uniprot
O75581 (LRP6) S1544 Uniprot
P01106 (MYC) T58 Uniprot
P05412 (JUN) T239 Uniprot
P05412 (JUN) S243 Uniprot
P05412 (JUN) S249 Uniprot
P10636-7 (MAPT) T152 Uniprot
P10636-7 (MAPT) S173 Uniprot
P10636-7 (MAPT) T176 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636-8 (MAPT) S235 Uniprot
P10636-8 (MAPT) S262 Uniprot
P10636-6 (MAPT) S266 Uniprot
P10636-8 (MAPT) S324 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636 (MAPT) S579 Uniprot
P10636 (MAPT) S641 Uniprot
P10636 (MAPT) S713 Uniprot
P10636 (MAPT) S721 Uniprot
P13807 (GYS1) S641 Uniprot
P17812 (CTPS1) S571 Uniprot
P20749 (BCL3) S402 Uniprot
P20749 (BCL3) S406 Uniprot
P22415 (USF1) T153 Uniprot
P22415 (USF1) S186 Uniprot
P23246 (SFPQ) T687 Uniprot
P24864 (CCNE1) T395 Uniprot
P31749 (AKT1) T312 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P35222 (CTNNB1) T41 Uniprot
P49840 (GSK3A) S278 Uniprot
P49840 (GSK3A) Y279 Uniprot
Q00613-1 (HSF1) S303 Uniprot
Q03060-16 (CREM) S83 Uniprot
Q05397 (PTK2) S722 Uniprot
Q07817 (BCL2L1) S62 Uniprot
Q07820-2 (MCL1) S159 Uniprot
Q07820-2 (MCL1) T163 Uniprot
Q13144 (EIF2B5) S540 Uniprot
Q13144 (EIF2B5) S544 Uniprot
Q13563 (PKD2) S76 Uniprot
Q16555 (DPYSL2) S518 Uniprot
Q16584 (MAP3K11) S789 Uniprot
Q16584 (MAP3K11) S793 Uniprot
Q2M1Z3 (ARHGAP31) T789 Uniprot
Q6R327 (RICTOR) T1695 Uniprot
Q8NHW3 (MAFA) S49 Uniprot
Q8NHW3 (MAFA) T53 Uniprot
Q8NHW3 (MAFA) T57 Uniprot
Q8NHW3 (MAFA) S61 Uniprot
Q92597 (NDRG1) S342 Uniprot
Q92597 (NDRG1) S352 Uniprot
Q92597 (NDRG1) S362 Uniprot
Q92908 (GATA6) S37 Uniprot
Q96RR4 (CAMKK2) S129 Uniprot
Q96RR4 (CAMKK2) S133 Uniprot
Q96RR4 (CAMKK2) S137 Uniprot
Q9BYG3 (NIFK) S230 Uniprot
Q9BYG3 (NIFK) T234 Uniprot
Q9NZ72 (STMN3) S60 Uniprot

Research Backgrounds


Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle (By similarity). May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (By similarity). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (By similarity). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer. Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity).


Phosphorylated by AKT1 at Ser-21: upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3A, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-279.

Subunit Structure:

Monomer. Interacts with ARRB2 (By similarity). Interacts with AXIN1 and CTNNB1/beta-catenin. Interacts with CTNND2. Interacts with LMBR1L. Interacts with DDX3X. Interacts with TNFRSF10B.


Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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