Product: XIAP Antibody
Catalog: AF6368
Description: Rabbit polyclonal antibody to XIAP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: P98170
RRID: AB_2835212

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
XIAP Antibody detects endogenous levels of total XIAP.
Cite Format: Affinity Biosciences Cat# AF6368, RRID:AB_2835212.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


AP 13; API3; Apoptosis Inhibitor 3; Baculoviral IAP repeat containing 4; Baculoviral IAP Repeat Containing Protein 4; Baculoviral IAP repeat-containing protein 4; BIRC 4; BIRC4; E3 ubiquitin-protein ligase XIAP; hIAP-3; hIAP3; HILP; IAP 3; IAP like protein; IAP-3; IAP-like protein; IAP3; ILP 1; ILP; ILP1; Inhibitor of apoptosis protein 3; Inhibitor of Apoptosis X Linked; Mammalian IAP Homologue A; MIHA; X linked IAP; X linked inhibitor of apoptosis; X linked inhibitor of apoptosis E3 ubiquitin protein ligase; X linked inhibitor of apoptosis protein; X-linked IAP; X-linked inhibitor of apoptosis protein; Xiap; XIAP_HUMAN; XLP2;



Ubiquitous, except peripheral blood leukocytes.

The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Similar to API1, BIRC4 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98170 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
K10 Ubiquitination
C12 S-Nitrosylation
K19 Ubiquitination
K31 Ubiquitination
T32 Phosphorylation
S40 Phosphorylation
S45 Phosphorylation
T46 Phosphorylation
S87 Phosphorylation P31751 (AKT2) , P31749 (AKT1)
C90 S-Nitrosylation
K116 Ubiquitination
Y120 Phosphorylation
S123 Phosphorylation
R131 Methylation
Y139 Phosphorylation
K168 Ubiquitination
T180 Phosphorylation
K208 Ubiquitination
C213 S-Nitrosylation
S218 Phosphorylation
R248 Methylation
K297 Ubiquitination
K299 Ubiquitination
C300 S-Nitrosylation
C303 S-Nitrosylation
K311 Ubiquitination
K322 Ubiquitination
C327 S-Nitrosylation
K328 Ubiquitination
K334 Ubiquitination
S347 Phosphorylation
C351 S-Nitrosylation
T355 Phosphorylation
T356 Phosphorylation
K358 Ubiquitination
S361 Phosphorylation
K387 Ubiquitination
K396 Ubiquitination
S400 Phosphorylation
S402 Phosphorylation
Y404 Phosphorylation
K405 Sumoylation
K405 Ubiquitination
S406 Phosphorylation
K419 Ubiquitination
T429 Phosphorylation
S430 Phosphorylation Q14164 (IKBKE)
K433 Ubiquitination
S436 Phosphorylation
T437 Phosphorylation
K448 Ubiquitination
T470 Phosphorylation
K480 Ubiquitination

Research Backgrounds


Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.


S-Nitrosylation down-regulates its E3 ubiquitin-protein ligase activity.

Autoubiquitinated and degraded by the proteasome in apoptotic cells.

Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: TLE3 promotes its nuclear localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous, except peripheral blood leukocytes.

Subunit Structure:

Monomer, and homodimer. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with TAB1/MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of TAB1/MAP3K7IP1 and AIFM1. Interacts with TCF25 and COMMD1. Interacts with SEPTIN4 isoform 6, but not with other SEPTIN4 isoforms. Interacts with RIP1, RIP2, RIP3, RIP4, CCS and USP19. Interacts (via BIR 2 domain and BIR 3 domain) with HAX1 (via C-terminus) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with the monomeric form of BIRC5/survivin. Interacts with TLE3 and TCF7L2/TCF4. Interacts (via BIR 3 and RING domains) with PDCL3.


The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit CASP3 and CASP7, while the third BIR is involved in CASP9 inhibition. The interactions with DIABLO/SMAC and PRSS25 are mediated by the second and third BIR domains.

Belongs to the IAP family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)


1). The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus. Animals, 2022 (PubMed: 36552491) [IF=3.0]

Application: WB    Species: Mouse    Sample: Phodopus sungorus

Figure 8. Levels of the Smad signaling pathway protein expression in the ovaries of Phodopus sungorus under three photoperiod conditions. (A,B) Representative immunoblots of the key SMAD4 and XIAP proteins and internal reference proteins (β-actin and GAPDH). (C,D) Relative protein expression of SMAD4 and XIAP. Values are means ± SEM. n = 8. SD, short daylight; MD, moderate daylight; LD, long daylight.

2). CircPAN3 mediates drug resistance in acute myeloid leukemia through the miR-153-5p/miR-183-5p-XIAP axis. EXPERIMENTAL HEMATOLOGY, 2019 (PubMed: 30395908) [IF=2.6]

Application: WB    Species: human    Sample: THP-1/ADM cells

Figure 5.| Panel (B) shows that the effects of circPAN3 down-regulation on XIAP expression and the ratios of CL Cas-3/pro-Cas-3 and CL Cas-9/pro-Cas-9 were blocked by specific inhibitors of miR-153-3p and miR-183-5p. The protein levels were determined by Western blot in THP-1/ADM cells co-transfected with circPAN3-specific siRNA and either miR-153-5p or miR-183-5p specific inhibitors.

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