Product: PKC theta Antibody
Catalog: AF6394
Description: Rabbit polyclonal antibody to PKC theta
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 80kDa; 82kD(Calculated).
Uniprot: Q04759
RRID: AB_2835229

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
PKC theta Antibody detects endogenous levels of total PKC theta.
Cite Format: Affinity Biosciences Cat# AF6394, RRID:AB_2835229.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


nPKC-theta; PRKCT; Protein kinase C theta; Protein kinase C theta type;



Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04759 As Substrate

Site PTM Type Enzyme
Y28 Phosphorylation
K48 Ubiquitination
K49 Ubiquitination
T51 Phosphorylation
K66 Ubiquitination
K77 Ubiquitination
Y90 Phosphorylation P06239 (LCK)
K110 Ubiquitination
Y121 Phosphorylation
K204 Ubiquitination
K208 Ubiquitination
T219 Phosphorylation Q04759 (PRKCQ)
K223 Ubiquitination
K227 Ubiquitination
K235 Ubiquitination
K240 Ubiquitination
T275 Phosphorylation
K276 Ubiquitination
K286 Ubiquitination
T307 Phosphorylation
S323 Phosphorylation
K325 Ubiquitination
K338 Ubiquitination
S348 Phosphorylation
K355 Ubiquitination
S370 Phosphorylation
K374 Ubiquitination
K376 Ubiquitination
K384 Acetylation
K384 Ubiquitination
K388 Ubiquitination
K400 Ubiquitination
K401 Ubiquitination
K409 Ubiquitination
K429 Ubiquitination
K506 Ubiquitination
K514 Ubiquitination
K527 Ubiquitination
K535 Ubiquitination
T536 Phosphorylation
T538 Phosphorylation Q13131 (PRKAA1) , Q8IVH8 (MAP4K3)
T542 Phosphorylation
Y545 Phosphorylation
K604 Ubiquitination
K607 Acetylation
K620 Ubiquitination
K645 Ubiquitination
K654 Ubiquitination
K656 Ubiquitination
S662 Phosphorylation
K666 Ubiquitination
K672 Ubiquitination
S676 Phosphorylation
S685 Phosphorylation
S695 Phosphorylation

PTMs - Q04759 As Enzyme

Substrate Site Source
O14920-1 (IKBKB) S177 Uniprot
O14920-1 (IKBKB) S181 Uniprot
O14974 (PPP1R12A) T853 Uniprot
O15111 (CHUK) S176 Uniprot
O15111 (CHUK) S180 Uniprot
O15530 (PDPK1) S501 Uniprot
O15530 (PDPK1) S529 Uniprot
O43516 (WIPF1) S488 Uniprot
O95267 (RASGRP1) T184 Uniprot
P01116-2 (KRAS) S181 Uniprot
P10588 (NR2F6) S83 Uniprot
P15311 (EZR) T567 Uniprot
P15407 (FOSL1) T223 Uniprot
P15407 (FOSL1) T230 Uniprot
P15407 (FOSL1) S265 Uniprot
P26038 (MSN) T558 Uniprot
P29474 (NOS3) T495 Uniprot
P32942 (ICAM3) S518 Uniprot
P35236 (PTPN7) S44 Uniprot
P35236 (PTPN7) S246 Uniprot
P35241 (RDX) T564 Uniprot
P35568 (IRS1) S1101 Uniprot
P41594-2 (GRM5) S840 Uniprot
Q04759 (PRKCQ) T219 Uniprot
Q09472 (EP300) S384 Uniprot
Q13191 (CBLB) S282 Uniprot
Q15052 (ARHGEF6) S225 Uniprot
Q15052 (ARHGEF6) S488 Uniprot
Q3V6T2-2 (CCDC88A) S1689 Uniprot
Q3V6T2 (CCDC88A) S1690 Uniprot
Q5JVS0 (HABP4) T354 Uniprot
Q5JVS0-1 (HABP4) T375 Uniprot
Q8IV61 (RASGRP3) T133 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q9BXL7 (CARD11) S559 Uniprot
Q9BXL7 (CARD11) S644 Uniprot
Q9BXL7 (CARD11) S652 Uniprot
Q9UEW8 (STK39) S309 Uniprot
Q9UEW8 (STK39) S323 Uniprot
Q9Y4G8 (RAPGEF2) S960 Uniprot

Research Backgrounds


Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylates CCDC88A/GIV and inhibits its guanine nucleotide exchange factor activity.


Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein.
Note: In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Subunit Structure:

Part of a lipid raft complex composed at least of BCL10, CARD11, MALT1 and IKBKB. Interacts with GLRX3 (via N-terminus). Interacts with ECT2. Interacts with CCDC88A/GIV; the interaction leads to phosphorylation of CCDC88A and inhibition of its guanine nucleotide exchange factor activity.


The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Measles.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.


1). Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36835236) [IF=5.6]

Application: WB    Species: Mouse    Sample: Mlg cells

Figure 5. Exogenous rhTβ4 inhibits pulmonary fibrosis and lung cancer via the IL6/JAK2/STAT3 signaling pathway. (A) The luciferase activity in the STAT3-SIE-luc-293 cell lysates treated with IL-6 (70 ng/mL) and rhTβ4. (B) The viability of rhTβ4-treated HEK-293 cells. (C) Western blot assay of JAK2, p-JAK2, STAT3 and p-STAT3 expression in rhTβ4-treated A549 cells. (D) Western blot assay of JAK2, p-JAK2, STAT3 and p-STAT3 expression in rhTβ4-treated Mlg cells. (E,F) Immunohistochemical staining analysis of p-JAK2 and p-STAT3 in mice. Scale bar = 50 μm. Data are shown as the mean ± SD.

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