CERS1 Antibody - #DF15450

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Product: CERS1 Antibody
Catalog: DF15450
Description: Rabbit polyclonal antibody to CERS1
Application: IF/ICC
Cited expt.:
Reactivity: Human, Mouse
Mol.Wt.: 40kD(Calculated).
Uniprot: P27544

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CERS1 Antibody detects endogenous levels of CERS1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ceramide synthase 1; CerS1; CERS1_HUMAN; LAG1; LAG1 homolog ceramide synthase 1; LAG1 longevity assurance homolog 1; LASS 1; LASS1; Longevity assurance (LAG1 S. cerevisiae) homolog 1; Longevity assurance gene 1; Longevity assurance gene 1 protein homolog 1; MGC90349; Protein LAG1; Protein UOG 1; Protein UOG-1; UOG 1 protein; UOG1; Upstream of GDF1;

Immunogens

Immunogen:

A synthesized peptide derived from human CERS1.

Uniprot:

P27544(CERS1_HUMAN) >>Visit HPA database.

Gene(ID):
CERS1(10715)
Sequence:
MAAAGPAAGPTGPEPMPSYAQLVQRGWGSALAAARGCTDCGWGLARRGLAEHAHLAPPELLLLALGALGWTALRSAATARLFRPLAKRCCLQPRDAAKMPESAWKFLFYLGSWSYSAYLLFGTDYPFFHDPPSVFYDWTPGMAVPRDIAAAYLLQGSFYGHSIYATLYMDTWRKDSVVMLLHHVVTLILIVSSYAFRYHNVGILVLFLHDISDVQLEFTKLNIYFKSRGGSYHRLHALAADLGCLSFGFSWFWFRLYWFPLKVLYATSHCSLRTVPDIPFYFFFNALLLLLTLMNLYWFLYIVAFAAKVLTGQVHELKDLREYDTAEAQSLKPSKAEKPLRNGLVKDKRF

Research Backgrounds

Function:

Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward stearoyl-CoA (octadecanoyl-CoA; C18:0-CoA).

PTMs:

Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Research Fields

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Exercise improves depressive-like behavior in adolescent mice by regulating sphingosine and ceramide metabolism through microglial CerS1. Communications biology, 2025 (PubMed: 40537488) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Fig. 3: CerS1 is upregulated in microglia of HIIT and MICT mice. A An experimental protocol for FACS and a gate strategy for CD11b+CD45Low microglia were presented. B, C Heat map of differentially expressed genes in microglia after HIIT and MICT. D, E KEGG analysis of the differentially expressed genes in HIIT and MICT. F CerS1 mRNA levels in microglia by QPCR analysis (N  = 3 per group, one-way ANOVA, F (3, 12) = 89.70). G Representative western blot images showing the protein level of CerS1 (N  = 3 per group, two-sided Student’s t tests, T (4) = 4.948). The mock treatment was a blank control with PBS added. Data are shown as the mean ± s.d. P  

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