Product: Cortactin Antibody
Catalog: AF6436
Description: Rabbit polyclonal antibody to Cortactin
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 85kDa; 62kD(Calculated).
Uniprot: Q14247
RRID: AB_2835261

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(91%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(91%), Dog(82%), Xenopus(80%)
Cortactin Antibody detects endogenous levels of total Cortactin.
Cite Format: Affinity Biosciences Cat# AF6436, RRID:AB_2835261.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Amplaxin; CTTN; EMS 1; EMS1; FLJ34459; Mammary tumor and squamous cell carcinoma associated; Oncogene EMS1; p80/85 src substrate; Src substrate cortactin; SRC8_HUMAN;


cortactin a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14247 As Substrate

Site PTM Type Enzyme
K3 Methylation
S11 Phosphorylation
T24 Phosphorylation
S33 Phosphorylation
K35 Ubiquitination
K42 Ubiquitination
T43 Phosphorylation
S47 Phosphorylation
K57 Ubiquitination
T68 Phosphorylation
K70 Methylation
K72 Methylation
K79 Methylation
K79 Ubiquitination
S81 Phosphorylation
Y84 Phosphorylation
K87 Acetylation
K87 Methylation
K87 Ubiquitination
S98 Phosphorylation
Y104 Phosphorylation
S106 Phosphorylation
K107 Methylation
K107 Ubiquitination
S109 Phosphorylation
K110 Ubiquitination
C112 S-Nitrosylation
S113 Phosphorylation
S117 Phosphorylation
R119 Methylation
K124 Acetylation
K124 Methylation
K124 Ubiquitination
R131 Methylation
S135 Phosphorylation
Y141 Phosphorylation
K144 Acetylation
K144 Sumoylation
K144 Ubiquitination
S150 Phosphorylation
K152 Ubiquitination
Y154 Phosphorylation
S155 Phosphorylation
S156 Phosphorylation
K161 Acetylation
K161 Methylation
K161 Ubiquitination
Y162 Phosphorylation
R168 Methylation
K171 Acetylation
K171 Ubiquitination
S172 Phosphorylation
Y178 Phosphorylation
K181 Acetylation
K181 Sumoylation
T182 Phosphorylation
S187 Phosphorylation
Y191 Phosphorylation
S192 Phosphorylation
K193 Ubiquitination
K198 Acetylation
K198 Ubiquitination
Y199 Phosphorylation
K203 Acetylation
K208 Ubiquitination
S209 Phosphorylation
Y215 Phosphorylation
K218 Acetylation
K218 Sumoylation
T219 Phosphorylation
S224 Phosphorylation
K226 Ubiquitination
Y228 Phosphorylation
K230 Ubiquitination
K235 Acetylation
K235 Methylation
K235 Ubiquitination
K245 Ubiquitination
S261 Phosphorylation P41743 (PRKCI)
K263 Ubiquitination
Y265 Phosphorylation
K266 Ubiquitination
T267 Phosphorylation
K272 Acetylation
K272 Methylation
K272 Ubiquitination
S277 Phosphorylation
S282 Phosphorylation
Y289 Phosphorylation
K290 Acetylation
K290 Ubiquitination
K295 Sumoylation
S298 Phosphorylation O14757 (CHEK1) , Q15139 (PRKD1)
Y302 Phosphorylation
S303 Phosphorylation
K304 Acetylation
K304 Ubiquitination
K309 Acetylation
K309 Methylation
Y310 Phosphorylation
K319 Acetylation
S322 Phosphorylation
T323 Phosphorylation
S331 Phosphorylation
S332 Phosphorylation
Y334 Phosphorylation P12931 (SRC)
K336 Ubiquitination
T337 Phosphorylation
S345 Phosphorylation
K346 Ubiquitination
S348 Phosphorylation Q15139 (PRKD1)
T399 Phosphorylation
T401 Phosphorylation
S405 Phosphorylation Q05655 (PRKCD) , P06493 (CDK1) , P28482 (MAPK1) , Q13153 (PAK1) , P24941 (CDK2) , P27361 (MAPK3)
T411 Phosphorylation
R414 Methylation
S417 Phosphorylation
S418 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , Q13153 (PAK1)
Y421 Phosphorylation P00519 (ABL1) , P42684 (ABL2)
S426 Phosphorylation
S432 Phosphorylation
Y433 Phosphorylation
S438 Phosphorylation
T440 Phosphorylation
Y446 Phosphorylation P12931 (SRC) , P11362 (FGFR1)
S447 Phosphorylation
Y453 Phosphorylation
Y464 Phosphorylation
Y470 Phosphorylation P42684 (ABL2) , P12931 (SRC) , P00519 (ABL1)
Y479 Phosphorylation
T485 Phosphorylation
Y486 Phosphorylation P42684 (ABL2) , P12931 (SRC) , P00519 (ABL1)
Y538 Phosphorylation
Y545 Phosphorylation
R549 Methylation

Research Backgrounds


Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane.


Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle. Cell projection>Dendrite. Cell projection. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Podosome. Cell junction. Cell junction>Focal adhesion. Membrane>Clathrin-coated pit. Cell projection>Dendritic spine. Cytoplasm>Cell cortex.
Note: Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SHANK2 and SHANK3 (via its SH3 domain). Also interacts with FGD1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with ABL2 (By similarity). Interacts with KCNA2 (via non-phosphorylated C-terminus). Interacts with PLXDC2 and SRCIN1. Interacts with SAMSN1 (via SH3 domain). Interacts (via SH3 domain) with ASAP1 (via Pro-rich region). Interacts (via SH3 domain) with DNM2 (By similarity). Interacts with ACTN1 (By similarity). Interacts with FER. Forms a complex made of ABL1 and MYLK. Interacts with CTTNBP2NL; this interaction may target CTTN to stress fibers. Interacts with CTTNBP2; this interaction may target CTTN at the cell cortex or dendritic spines. Interacts with KCNH1.


The SH3 motif may mediate binding to the cytoskeleton.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

Restrictive clause


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