XPF Antibody | Affinity Biosciences

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XPF Antibody - Western blot analysis of extracts from rat heart, using XPF Antibody.

Product:	XPF Antibody
Catalog:	AF0295
Description:	Rabbit polyclonal antibody to XPF
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	103kDa; 104kD(Calculated).
Uniprot:	Q92889
RRID:	AB_2833461

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

XPF Antibody detects endogenous levels of total XPF.

RRID:

AB_2833461
Cite Format: Affinity Biosciences Cat# AF0295, RRID:AB_2833461.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA excision repair protein ERCC 4; DNA excision repair protein ERCC-4; DNA excision repair protein ERCC4; DNA repair endonuclease XPF; DNA repair protein complementing XP F cells; DNA repair protein complementing XP-F cells; ERCC 11; ERCC 4; ERCC11; ERCC4; Excision repair complementing defective in Chinese hamster; Excision repair cross complementing rodent repair deficiency complementation group 4; excision repair cross-complementation group 4; FANCQ; RAD 1; RAD1; Xeroderma pigmentosum complementation group F; Xeroderma pigmentosum group F complementing protein; Xeroderma pigmentosum group F-complementing protein; Xeroderma pigmentosum VI; XP, group G; XP6; XPF_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human XPF, corresponding to a region within C-terminal amino acids.

Uniprot:

Q92889(XPF_HUMAN) >>Visit HPA database.

Gene(ID):

ERCC4(2072)

Description:

ERCC4 Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. Belongs to the XPF family. Heterodimer composed of ERCC1 and XPF/ERCC4.

Sequence:

Q92889 XPF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESGQPARRIAMAPLLEYERQLVLELLDTDGLVVCARGLGADRLLYHFLQLHCHPACLVLVLNTQPAEEEYFINQLKIEGVEHLPRRVTNEITSNSRYEVYTQGGVIFATSRILVVDFLTDRIPSDLITGILVYRAHRIIESCQEAFILRLFRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPTMLAIQTAILDILNACLKELKCHNPSLEVEDLSLENAIGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKILRTLLQYLSQYDCVTFLNLLESLRATEKAFGQNSGWLFLDSSTSMFINARARVYHLPDAKMSKKEKISEKMEIKEGEETKKELVLESNPKWEALTEVLKEIEAENKESEALGGPGQVLICASDDRTCSQLRDYITLGAEAFLLRLYRKTFEKDSKAEEVWMKFRKEDSSKRIRKSHKRPKDPQNKERASTKERTLKKKKRKLTLTQMVGKPEELEEEGDVEEGYRREISSSPESCPEEIKHEEFDVNLSSDAAFGILKEPLTIIHPLLGCSDPYALTRVLHEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASMVVPEEREGRDETNLDLVRGTASADVSTDTRKAGGQEQNGTQQSIVVDMREFRSELPSLIHRRGIDIEPVTLEVGDYILTPEMCVERKSISDLIGSLNNGRLYSQCISMSRYYKRPVLLIEFDPSKPFSLTSRGALFQEISSNDISSKLTLLTLHFPRLRILWCPSPHATAELFEELKQSKPQPDAATALAITADSETLPESEKYNPGPQDFLLKMPGVNAKNCRSLMHHVKNIAELAALSQDELTSILGNAANAKQLYDFIHTSFAEVVSKGKGKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Bovine

Sheep

Chicken

Rabbit

Xenopus

Horse

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q92889_XPF_HUMAN

Function:

Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the XPF family.

Research Fields

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

Restrictive clause

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For Research Use Only.
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XPF Antibody - #AF0295