Product: FANCD2 Antibody
Catalog: AF6480
Description: Rabbit polyclonal antibody to FANCD2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 166kDa; 164kD(Calculated).
Uniprot: Q9BXW9
RRID: AB_2835299

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(100%), Xenopus(83%)
FANCD2 Antibody detects endogenous levels of total FANCD2.
Cite Format: Affinity Biosciences Cat# AF6480, RRID:AB_2835299.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


DKFZp762A223; FA 4; FA D2; FA4; FAC D2; FACD 2; FACD; FACD2; FACD2_HUMAN; FAD; FAD2; FANC D2; FANCD 2; FANCD; FANCD2; FANCONI ANEMIA COMPLEMENTATION GROUP D; Fanconi anemia complementation group D2; Fanconi anemia group D2 protein; FANCONI PANCYTOPENIA TYPE 4; FLJ23826; OTTHUMP00000158853; OTTHUMP00000207925; Protein FACD2; Type 4 Fanconi pancytopenia;



Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.

FANCD2 Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXW9 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S15 Phosphorylation
K33 Ubiquitination
K34 Ubiquitination
K50 Ubiquitination
K53 Ubiquitination
K60 Ubiquitination
K77 Ubiquitination
K78 Ubiquitination
K133 Ubiquitination
K156 Ubiquitination
K165 Ubiquitination
S167 Phosphorylation
S178 Phosphorylation
K181 Ubiquitination
K190 Ubiquitination
S201 Phosphorylation
S222 Phosphorylation Q13315 (ATM)
S251 Phosphorylation
K261 Ubiquitination
S271 Phosphorylation
S272 Phosphorylation
S319 Phosphorylation
K322 Ubiquitination
S331 Phosphorylation O14757 (CHEK1)
K358 Ubiquitination
K366 Ubiquitination
Y458 Phosphorylation
K561 Ubiquitination
K569 Ubiquitination
S590 Phosphorylation
S592 Phosphorylation
S594 Phosphorylation
T596 Phosphorylation Q13535 (ATR)
T608 Phosphorylation Q13535 (ATR)
Y684 Phosphorylation
T691 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S717 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
K723 Ubiquitination
K878 Ubiquitination
S886 Phosphorylation
K889 Ubiquitination
T896 Phosphorylation
K905 Ubiquitination
K913 Ubiquitination
S978 Phosphorylation
T981 Phosphorylation
K992 Methylation
K992 Ubiquitination
K1154 Ubiquitination
K1172 Acetylation
K1198 Acetylation
S1214 Phosphorylation
K1216 Ubiquitination
K1248 Ubiquitination
T1253 Phosphorylation
S1257 Phosphorylation Q13315 (ATM)
K1346 Ubiquitination
K1361 Ubiquitination
K1362 Ubiquitination
T1363 Phosphorylation
K1372 Ubiquitination
K1390 Ubiquitination
S1401 Phosphorylation Q13315 (ATM)
S1404 Phosphorylation Q13315 (ATM)
S1407 Phosphorylation Q13315 (ATM)
T1408 Phosphorylation
S1412 Phosphorylation
S1418 Phosphorylation
S1423 Phosphorylation
T1426 Phosphorylation
S1435 Phosphorylation

Research Backgrounds


Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.


Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.

Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.

Subcellular Location:

Note: Concentrates in nuclear foci during S phase and upon genotoxic stress. At the onset of mitosis, excluded from chromosomes and diffuses into the cytoplasm, returning to the nucleus at the end of cell division. Observed in a few spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites and could be sites of replication fork stalling. The foci are frequently interlinked through BLM-associated ultra-fine DNA bridges. Following aphidicolin treatment, targets chromatid gaps and breaks.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.

Subunit Structure:

Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1 and BLM. Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3. The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage. Interacts with DCLRE1B/Apollo. Interacts with POLN.


The C-terminal 24 residues of isoform 2 are required for its function.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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