Product: Cox1 Antibody
Catalog: AF7002
Description: Rabbit polyclonal antibody to Cox1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Pig
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 70kDa; 69kD(Calculated).
Uniprot: P23219
RRID: AB_2835310

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 50ul $250 In stock
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 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig
Prediction:
Horse(92%), Rabbit(82%), Dog(83%)
Clonality:
Polyclonal
Specificity:
Cox1 Antibody detects endogenous levels of total Cox1.
RRID:
AB_2835310
Cite Format: Affinity Biosciences Cat# AF7002, RRID:AB_2835310.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COX 1; COX 3; COX-1; COX1; Cox3; Cyclooxygenase 1; Cyclooxygenase 3, included; Cyclooxygenase-1; EC 1.14.99.1; Partial COX1 proteins, included; PCOX1; PGG/HS; PGH synthase 1; PGH1_HUMAN; PGHS-1; PGHS1; PHS 1; PHS1; Prostaglandin G/H synthase 1; Prostaglandin H2 synthase 1; Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin-endoperoxide synthase 1; PTGHS; PTGS1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
COX-1 May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
MSRSLLLWFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQCDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWEFVNATFIREMLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGKLKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLYATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQLSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQEYSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDVIRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFGGEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Pig
83
Dog
83
Rabbit
82
Sheep
67
Bovine
58
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23219 As Substrate

Site PTM Type Enzyme
Y54 Phosphorylation
T117 Phosphorylation
T220 Phosphorylation

Research Backgrounds

Function:

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

Subcellular Location:

Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the prostaglandin G/H synthase family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Urolithin A protects severe acute pancreatitis-associated acute cardiac injury by regulating mitochondrial fatty acid oxidative metabolism in cardiomyocytes. MedComm, 2023 (PubMed: 38116065) [IF=9.9]

2). Integrin 51, as a Receptor of Fibronectin, Binds the FbaA Protein of Group A Streptococcus To Initiate Autophagy during Infection. mBio, 2020 (PubMed: 32518187) [IF=6.4]

Application: WB    Species: Mouse    Sample: Hep2 cells

FIG 6 FbaA initiates autophagy upon Fn-mediated binding to integrin 51. (A and B) Western blot analysis of TLR2 (A) and TLR4 (B) expression in Hep2 cells stimulated with FbaA for the indicated times. (C and D) Expression of LC3II in Hep2 cells with and without TLR2- or TLR4-specific siRNA following FbaA stimulation. (E) Schematic representation of the Fn subunit. Fn contains domain types I, II, and III. FnI is the region critical for binding to FnBP; FnIII9 and FnIII10 contain motifs that interact with integrins. (F) Hep2 cells were transfected with GST-FbaA, GST-FbaAFn, or GST (as a control), and the integrin 5 or 1 chain was measured after being pulled down with anti-GST Ab. (G) Expression of Fn, integrin 5, integrin 1, and LC3II in Hep2 cells stimulated with FbaA at the indicated concentrations, as analyzed by Western blotting. (H) Western blot analysis of LC3II expression in Hep2 cells stimulated with different concentrations of Fn and mock-FbaA or FbaA. (I to K) Expression of LC3II in Hep2 cells with or without specific siRNA against Fn, integrin 5, or integrin 1 following FbaA stimulation, as analyzed by Western blotting. (L and M) Fn, integrin 5, and integrin 1 knockdown cells were pretreated without (L) or with (M) rapamycin for 0.5 h and then infected with M1 GAS strain SF370 for 6 h. Numbers of CFU of M1 GAS strain SF370 were counted in the cells. (N) M1 GAS strain SF370-infected sh-Atg5-Hep2 cells with specific siRNA against Fn, integrin 5, or integrin 1. Numbers of CFU of M1 GAS strain SF370 were counted in the cells. *, P  0.05.

Application: WB    Species: human    Sample: Hep2 cells

FIG 6 |FbaA initiates autophagy upon Fn-mediated binding to integrin 51. (A and B) Western blot analysis of TLR2 (A) and TLR4 (B) expression in Hep2 cells stimulated with FbaA for the indicated times.

3). All That Glitters Is Not Gold: Assessment of Bee Pollen Supplementation Effects on Gastric Mucosa. Nutrients, 2023 (PubMed: 38201868) [IF=5.9]

4). Aspirin in combination with gastrodin protects cardiac function and mitigates gastric mucosal injury in response to myocardial ischemia/reperfusion. Frontiers in Pharmacology, 2022 (PubMed: 36238560) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 1 Aspirin inhibits myocardial inflammation and alleviates cardiac dysfunction in response to I/R injury. (A) Representative electrocardiogram shows the successful establishment of myocardial I/R injury model (n = 5 per group). The red arrows represent changes in the ST segment. (B) Representative images show TTC staining of the myocardium from rats (n = 5 per group) belonging to the sham, I/R, and I/R + 50 mg/kg aspirin groups. The infarct size quantification of the 3 groups of rats is also shown. (C) ELISA assay results show the serum levels of creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT) in the sham, I/R, and I/R + aspirin group rats (n = 5 per group). (D) Laggendorff in vitro cardiac perfusion results show the left ventricular developed pressure (LVDP), maximum and minimum rates of pressure development (max dP/dt and min dP/dt), and heart rate (HR) in the sham, I/R, and I/R + aspirin group rats (n = 5 per group). (E) Representative H&E staining images (×200 and ×400) show the histological details of the myocardium from the sham, I/R, and I/R + aspirin group rats (n = 5 per group). (F,G) Representative immunoblots and quantification of the expression levels of COX-1 and COX-2 in the sham, I/R, and I/R + aspirin group rats (n = 5 per group). (H) ELISA results show the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) level in the myocardial tissues of the sham, I/R, and I/R + aspirin group rats (n = 5 per group). The tissues were harvested 2 h post-reperfusion. The data are represented as means ± SD; *p < 0.05 vs. the sham group; # p < 0.05 vs. the I/R group.

Application: IF/ICC    Species: Rat    Sample:

FIGURE 3 Gasrtodin alleviates dual gastric mucosal injury by suppressing inflammation. (A) Representative images of the harvest stomach specimens from the sham, I/R, I/R + aspirin, I/R + 50 mg/kg aspirin + 200 mg/kg gastrodin group, and I/R + 200 mg/kg gastrodin group rats (n = 5 per group). (B) Byron gastric mucosa injury scores of the sham, I/R, I/R + aspirin, I/R + aspirin + gastrodin group, and I/R + gastrodin group rats (n = 5 per group). The scoring criteria are shown in Table 1. (C) Representative images (×100 and ×200) of the H&E-staining gastric tissue sections from the sham, I/R, I/R + aspirin, I/R + aspirin + gastrodin group, and I/R + gastrodin group rats (n = 5 per group). (D) Representative immunofluorescence staining images (×200) show the COX-1 and COX-2 expression levels in the gastric tissues of the sham, I/R, I/R + aspirin, I/R + aspirin + gastrodin group, and I/R + gastrodin group rats (n = 5 per group). The green denotes COX-1, red denotes COX-2, and blue denotes the nucleus. (E–G) ELISA assay results show the levels of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE2) and endothelin (ET) in the gastric tissues of the sham, I/R, I/R + aspirin, I/R + aspirin + gastrodin group,and I/R + gastrodin group rats (n = 5 per group). The data are represented as means ± SD; * p < 0.05 vs. the sham group; # p < 0.05 vs. the I/R group; ^ p < 0.05 vs. the I/R + aspirin group; & p < 0.05 vs. the I/R + aspirin + gastrodin group.

5). Mechanisms of pancreatic tumor suppression mediated by Xiang-lian pill: An integrated in silico exploration and experimental validation. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35931303) [IF=5.4]

6). Modulating Endogenous Oxygen Consumption Enhanced AIEgens-Mediated Photodynamic Therapy against Advanced Bladder Tumor. Particle & Particle Systems Characterization, 2021 [IF=2.7]

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