Product: Cox2 Antibody
Catalog: AF7003
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 80kD, 70 kD; 69kD(Calculated).
Uniprot: P35354
RRID: AB_2835311

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Cox2 Antibody detects endogenous levels of total Cox2.
Cite Format: Affinity Biosciences Cat# AF7003, RRID:AB_2835311.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase; Cyclooxygenase-2; Cyclooxygenase2; EC; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524; PES-2; PGG/HS; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2; ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10;


COX-2 Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35354 As Substrate

Site PTM Type Enzyme
N53 N-Glycosylation
Y120 Phosphorylation P07948 (LYN)
N130 N-Glycosylation
K155 Ubiquitination
K166 Ubiquitination
K346 Ubiquitination
N396 N-Glycosylation
K445 Ubiquitination
Y446 Phosphorylation P06241 (FYN)
N580 N-Glycosylation

Research Backgrounds


Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia (By similarity).


S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.

Subcellular Location:

Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:



Belongs to the prostaglandin G/H synthase family.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.


1). Fan X et al. Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation. Eur J Med Chem 2020 May 1;193:112217. (PubMed: 32182488) [IF=7.088]

Application: WB    Species: Mouse    Sample: BV2 and C6 cells

Fig. 2. Effects of compounds on protein expressions of iNOS, COX-2 in LPS-induced BV2 cell lines (A) and effects of compounds on protein expressions of iNOS in LPS-induced C6 cell lines (B). BV2 cell lines were exposed to the indicated compounds at concentrations of 10, 20 and 40 mM for 24 h in the presence of LPS (1 mg/mL). C6 cell lines were exposed to the indicated compounds at concentrations of 10, 20 and 40 mM for 24 h in the presence of LPS (10 mg/mL). The expression of iNOS and COX-2 was assayed and calculated by image J. Each error bar represents the mean ± SD of three independent experiments. #, p < 0.05 versus the control group; ##, p < 0.01 versus the control group; ###, p < 0.001 versus the control group. *, p < 0.05, **, p < 0.01 when compared to the LPS-treated cells. All data are the mean ± SD of at least three independent experiments.

2). Xian S et al. The Protective Effect of Evodiamine in Osteoarthritis: An In Vitro and In Vivo Study in Mice Model. Front Pharmacol 2022 Jun 20;13:899108. (PubMed: 35795554) [IF=5.810]

3). Lu J et al. Polysaccharides From the Aerial Parts of Tetrastigma Hemsleyanum Diels et Gilg Induce Bidirectional Immunity and Ameliorate LPS-Induced Acute Respiratory Distress Syndrome in Mice. Front Pharmacol 2022 Mar 11;13:838873. (PubMed: 35370633) [IF=5.810]

Application: WB    Species: mouse    Sample: RAW264.7 cells

FIGURE 3 | Effects of SYQP on the release of inflammatory cytokines and Oxidative stress in LPS-induced RAW264.7 cells.(F–J) RAW264.7 cells were treated in previous mentioned ways, and the gene and protein expression of iNOS and COX-2 were examined.

4). Zhang YX et al. Antidepressant-like effects of helicid on a chronic unpredictable mild stress-induced depression rat model: Inhibiting the IKK/IκBα/NF-κB pathway through NCALD to reduce inflammation. Int Immunopharmacol 2021 Apr;93:107165. (PubMed: 33578182) [IF=5.714]

Application: WB    Species: Rat    Sample: hippocampus

Fig. 7. Changes of inflammatory factors in the hippocampus after AAV-siNCALD using western blotting. (A) Representative iNOS, COX-2, TNF-α, IL-1β, IL-6, and β-actin bands. (B), (C), (D), (E), (F) The relative ratios of grayscale values of iNOS, COX-2, TNF-α, IL-1β, and IL-6 to that of corresponding β-actin, n = 4. ###P < 0.001, CUMS + saline vs. sham; *P < 0.05; **P < 0.01; ***P < 0.001, CUMS + saline vs. CUMS + AAV-siNCALD. All data are shown as the mean ± SEM.

5). Zhang Y et al. 6′-O-Galloylpaeoniflorin attenuates Helicobacter pylori-associated gastritis via modulating Nrf2 pathway. Int Immunopharmacol 2022 Aug 11;111:109122. (PubMed: 35964411) [IF=5.714]

6). Jiao J et al. Mechanisms of pancreatic tumor suppression mediated by Xiang-lian pill: An integrated in silico exploration and experimental validation. J Ethnopharmacol 2022 Nov 15;298:115586. (PubMed: 35931303) [IF=5.195]

7). Cui Q et al. A network pharmacology approach to investigate the mechanism of Shuxuening injection in the treatment of ischemic stroke. J Ethnopharmacol 2020 Apr 18:112891 (PubMed: 32315738) [IF=5.195]

Application: WB    Species: rat    Sample: hippocampal

Fig. 13. |Expression of protein of three genes detected through Western blot. Note: Sham vs I/R, *P < 0.05; SXNI vs I/R, #P < 0.05.

8). Zhang H et al. Mst2 Overexpression Inhibits Thyroid Carcinoma Growth and Metastasis by Disrupting Mitochondrial Fitness and Endoplasmic Reticulum Homeostasis. J Oncol 2021 Sep 6;2021:1262291. (PubMed: 34557228) [IF=4.501]

9). Liu R et al. Ibuprofen Exerts Antiepileptic and Neuroprotective Effects in the Rat Model of Pentylenetetrazol-Induced Epilepsy via the COX-2/NLRP3/IL-18 Pathway. Neurochem Res 2020 Aug 13. (PubMed: 32789796) [IF=4.414]

Application: IHC    Species: Rat    Sample: brain tissue

Fig. 3 Immunohistochemistry showing the expression of COX-2 in the brain tissue of rats in each group. a Immunohistochemistry of COX-2 in the CA1 area of the hippocampus. Calibration bars: 50 μm. b The comparison of the mean optical density (MOD) of COX-2 in diferent groups (*P<0.05, **P<0.01; one-way ANOVA)

Application: WB    Species: Rat    Sample: brain tissue

Fig. 4 Western blot for COX-2 in the brain tissue of rats in each group (*P<0.05, **P<0.01; one-way ANOVA)

10). Hu Y et al. Coixol Suppresses NF-κB, MAPK Pathways and NLRP3 Inflammasome Activation in Lipopolysaccharide-Induced RAW 264.7 Cells. Molecules 2020 Feb 18;25(4) (PubMed: 32085388) [IF=4.411]

Application: WB    Species: Mice    Sample: RAW 264.7 cells

Figure 3 Effects of coixol on the expression of proinflammatory mediators in LPS-induced RAW 264.7 cells. Cells were pretreated with coixol for 4 h and then stimulated with 1μg/mL LPS for 4 h. (A) The production of nitric oxide (NO) in cells supernatant. NO was measured by Griess assay. The protein levels of (B) inducible-nitric oxide synthase (iNOS) and (C) cyclooxygenase (COX)-2. Protein levels were measured by western blotting. GAPDH was used as internal control. The data represent the mean ± SD (NO, n = 6; iNOS, COX-2, n = 3). * p < 0.05 vs. control group. # p < 0.05 vs. LPS group.

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