Product: Ku70 Antibody
Catalog: AF0300
Description: Rabbit polyclonal antibody to Ku70
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 70kDa; 70kD(Calculated).
Uniprot: P12956
RRID: AB_2833465

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Ku70 antibody detects endogenous levels of total Ku70.
RRID:
AB_2833465
Cite Format: Affinity Biosciences Cat# AF0300, RRID:AB_2833465.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5''-deoxyribose-5-phosphate lyase Ku70; 5''-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku p70; Ku70; Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid autoantigen; Thyroid lupus autoantigen; Thyroid lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6; XRCC 6; Xrcc6; XRCC6_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Ku70

Uniprot:
Gene(ID):
Description:
Ku70 a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
Sequence:
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

PTMs - P12956 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S6 Phosphorylation P78527 (PRKDC)
Y7 Phosphorylation
Y8 Phosphorylation
K9 Sumoylation
S27 Phosphorylation P78527 (PRKDC) , Q13315 (ATM)
Y30 Phosphorylation
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
S33 Phosphorylation Q13315 (ATM) , P78527 (PRKDC)
S37 Phosphorylation
S45 Phosphorylation
S51 Phosphorylation P78527 (PRKDC)
S53 Phosphorylation
K92 Acetylation
K92 Methylation
K92 Ubiquitination
K94 Ubiquitination
K100 Acetylation
K100 Ubiquitination
Y103 Phosphorylation
K114 Acetylation
K114 Methylation
K114 Ubiquitination
K123 Methylation
K123 Sumoylation
K123 Ubiquitination
K129 Ubiquitination
S155 Phosphorylation
S180 Phosphorylation
K182 Ubiquitination
K189 Ubiquitination
R194 Methylation
K206 Ubiquitination
K207 Methylation
K207 Ubiquitination
S222 Phosphorylation
R230 Methylation
S237 Phosphorylation
K238 Ubiquitination
R244 Methylation
K265 Ubiquitination
K282 Acetylation
K282 Ubiquitination
K287 Acetylation
K287 Ubiquitination
T292 Phosphorylation
K297 Sumoylation
K297 Ubiquitination
K299 Ubiquitination
R301 Methylation
T302 Phosphorylation
S306 Phosphorylation
S314 Phosphorylation
K317 Acetylation
K317 Sumoylation
K317 Ubiquitination
R318 Methylation
S324 Phosphorylation
K331 Acetylation
K331 Sumoylation
K331 Ubiquitination
K338 Acetylation
K338 Ubiquitination
K351 Ubiquitination
K357 Ubiquitination
K358 Ubiquitination
K392 Acetylation
K392 Ubiquitination
K424 Sumoylation
T428 Phosphorylation
K443 Sumoylation
K443 Ubiquitination
K445 Ubiquitination
K451 Ubiquitination
T455 Phosphorylation P24941 (CDK2)
K461 Acetylation
K461 Ubiquitination
K463 Ubiquitination
K468 Acetylation
K468 Ubiquitination
R474 Methylation
S475 Phosphorylation
S477 Phosphorylation O14757 (CHEK1)
K510 Sumoylation
K516 Ubiquitination
S520 Phosphorylation
K526 Sumoylation
K526 Ubiquitination
Y530 Phosphorylation P12931 (SRC)
Y534 Phosphorylation
K539 Acetylation
K539 Ubiquitination
T541 Phosphorylation
K542 Acetylation
K544 Acetylation
S550 Phosphorylation
K553 Acetylation
K553 Ubiquitination
K556 Acetylation
K556 Sumoylation
K556 Ubiquitination
S560 Phosphorylation
K565 Ubiquitination
K570 Acetylation
K570 Ubiquitination
K575 Acetylation
K575 Ubiquitination
T577 Phosphorylation
K582 Ubiquitination
Y588 Phosphorylation
K591 Ubiquitination
S592 Phosphorylation
K595 Ubiquitination
K596 Ubiquitination
T604 Phosphorylation
K605 Acetylation
K605 Ubiquitination

Research Backgrounds

Function:

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

PTMs:

Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.

ADP-ribosylated by PARP3.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interacts with ATP23. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Binds to CDK9 isoform 2. Identified in a complex with DEAF1 and XRCC5. Interacts with DEAF1 (via the SAND domain); the interaction is direct and may be inhibited by DNA-binding. Interacts with CLU (By similarity). Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation. Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts with HMBOX1.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

Family&Domains:

Belongs to the ku70 family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

References

1). Quercetin inhibits DNA damage responses to induce apoptosis via SIRT5/PI3K/AKT pathway in non-small cell lung cancer. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37390710) [IF=7.5]

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