Product: ADAM 17 Antibody
Catalog: AF6361
Description: Rabbit polyclonal antibody to ADAM 17
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 93 kDa; 93kD(Calculated).
Uniprot: P78536
RRID: AB_2835372

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
ADAM 17 Antibody detects endogenous levels of total ADAM 17.
Cite Format: Affinity Biosciences Cat# AF6361, RRID:AB_2835372.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); A disintegrin and metalloproteinase domain 17; ADA17_HUMAN; ADAM 17; ADAM metallopeptidase domain 17; ADAM17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; Disintegrin and metalloproteinase domain-containing protein 17; MGC71942; NISBD; NISBD1; Snake venom like protease; Snake venom-like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme;


P78536 ADA17_HUMAN:

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

This gene encodes a disintegrin and metalloprotease (ADAM) domain 17, which is a member of the ADAM protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. This gene contains 19 exons and alternative splicing generates 2 transcripts.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78536 As Substrate

Site PTM Type Enzyme
Y82 Phosphorylation
T84 Phosphorylation
T87 Phosphorylation
K102 Ubiquitination
K168 Ubiquitination
K182 Ubiquitination
K197 Ubiquitination
K302 Ubiquitination
K309 Ubiquitination
K376 Ubiquitination
Y379 Phosphorylation
S382 Phosphorylation
K388 Ubiquitination
K392 Ubiquitination
Y433 Phosphorylation
Y436 Phosphorylation
K460 Ubiquitination
K465 Ubiquitination
K620 Ubiquitination
S706 Phosphorylation
K728 Ubiquitination
T735 Phosphorylation P27361 (MAPK3) , P17252 (PRKCA) , Q16539 (MAPK14) , P28482 (MAPK1) , Q05655 (PRKCD) , Q02156 (PRKCE)
S747 Phosphorylation
K753 Ubiquitination
T761 Phosphorylation
S767 Phosphorylation
T768 Phosphorylation
S770 Phosphorylation
K779 Ubiquitination
S785 Phosphorylation
K790 Ubiquitination
S791 Phosphorylation
T796 Phosphorylation
K805 Ubiquitination
S808 Phosphorylation
K810 Ubiquitination
S819 Phosphorylation

Research Backgrounds


Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity).


The precursor is cleaved by a furin endopeptidase.

Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

Subunit Structure:

Interacts with MAD2L1, MAPK14 and MUC1. Interacts with iRhom1/RHBDF1 and iRhom2/RHBDF2. Interacts with FRMD8 via its interaction with iRhom1/RHBDF1 and iRhom2/RHBDF2.


Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR (By similarity).

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.


1). Zhou J et al. MicroRNA-145 overexpression attenuates apoptosis and increases matrix synthesis in nucleus pulposus cells. LIFE SCIENCES 2019 Feb 20 (PubMed: 30797016) [IF=6.1]

Application: WB    Species: human    Sample: NP cells

Fig. 6. |ADAM17 suppression enhanced matrix metabolism in NP cells.(A,B) Western blotting (A) and subsequent densitometric analysis (B) revealed that collagen IIa and aggrecan protein expression in rat NP cells was increased by the ADAM17 inhibitor. GAPDH was used as the control. (C) Immunofluorescence imaging of human NP cells treated with LV-shControl and LV-shADAM17. (D, E)Western blotting (D) and subsequent densitometric analysis (E) demonstrated that ADAM17 suppression by LV-shADAM17 markedly increased collagen IIa and aggrecan protein expression in human NP cells.

Restrictive clause


Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.