AFfirm™ ENaC gamma Mouse Monoclonal Antibody - #BF9433
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
Amiloride sensitive epithelial sodium channel gamma subunit; Amiloride sensitive sodium channel subunit gamma; Amiloride-sensitive sodium channel subunit gamma; BESC3; ENaC gamma subunit; ENaCG; ENaCgamma; Epithelial Na(+) channel subunit gamma; Epithelial Na+ channel subunit gamma; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage gated sodium channel 1 subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; PHA1; SCNEG; SCNN 1G; SCNN1G; SCNNG_HUMAN; Sodium channel non voltage gated 1 gamma subunit; Sodium channel nonvoltage gated 1 gamma;
Immunogens
- P51170 SCNNG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYCLNTNTHGCRRIVVSRGRLRRLLWIGFTLTAVALILWQCALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQETREALKSLYGFPESRKRREAESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKRKVGGSIIHKASNVMHIESKQVVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWLLPVLTWDQGRQVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDDDLPTFNSALHLPPALGTQVPGTPPPKYNTLRLERAFSNQLTDTQMLDEL
PTMs - P51170 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| T98 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| K495 | Acetylation | Uniprot | |
| K498 | Acetylation | Uniprot | |
| K503 | Acetylation | Uniprot | |
| T622 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| T629 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| T641 | Phosphorylation | Uniprot |
Research Backgrounds
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.
Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.
ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.
Apical cell membrane>Multi-pass membrane protein.
Note: Apical membrane of epithelial cells.
Expressed in kidney (at protein level).
Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4; via the WW domains. Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full-length immature form of PCSK9 (pro-PCSK9).
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.
Research Fields
· Organismal Systems > Sensory system > Taste transduction.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
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