Product: HGS Antibody
Catalog: AF0316
Description: Rabbit polyclonal antibody to HGS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 86kDa; 86kD(Calculated).
Uniprot: O14964
RRID: AB_2833480

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
HGS Antibody detects endogenous levels of total HGS.
Cite Format: Affinity Biosciences Cat# AF0316, RRID:AB_2833480.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Hepatocyte growth factor regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; HGF-regulated tyrosine kinase substrate; HGNC:4897; HGS; HGS_HUMAN; Hrs; Human growth factor regulated tyrosine kinase substrate; Protein pp110; ZFYVE8;



Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Hrs a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14964 As Substrate

Site PTM Type Enzyme
K43 Ubiquitination
K56 Ubiquitination
K86 Ubiquitination
K93 Ubiquitination
K97 Ubiquitination
Y125 Phosphorylation
K126 Ubiquitination
T131 Phosphorylation
Y132 Phosphorylation
K136 Ubiquitination
K146 Ubiquitination
K192 Ubiquitination
K196 Ubiquitination
K202 Ubiquitination
K207 Acetylation
K207 Ubiquitination
Y216 Phosphorylation
K222 Ubiquitination
K226 Ubiquitination
T228 Phosphorylation
Y237 Phosphorylation
T239 Phosphorylation
S240 Phosphorylation
S243 Phosphorylation
S246 Phosphorylation
K251 Ubiquitination
K277 Ubiquitination
K283 Ubiquitination
S284 Phosphorylation
Y286 Phosphorylation
T287 Phosphorylation
S288 Phosphorylation
Y289 Phosphorylation
K291 Ubiquitination
S305 Phosphorylation
S306 Phosphorylation
Y308 Phosphorylation
S309 Phosphorylation
S310 Phosphorylation
Y329 Phosphorylation P00533 (EGFR)
Y334 Phosphorylation P00533 (EGFR)
K337 Ubiquitination
S345 Phosphorylation
T347 Phosphorylation
T355 Phosphorylation
Y466 Phosphorylation
Y467 Phosphorylation
K473 Ubiquitination
S485 Phosphorylation
K494 Acetylation
K513 Ubiquitination
K521 Ubiquitination
Y524 Phosphorylation
K541 Ubiquitination
K551 Ubiquitination
Y567 Phosphorylation

Research Backgrounds


Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.


Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.

Ubiquitinated. Ubiquitinated by ITCH.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Endosome>Multivesicular body membrane>Peripheral membrane protein.
Note: Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Subunit Structure:

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2 (By similarity). Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25 (By similarity). Identified in a complex with STAM and LITAF. Found in a complex with STAM and E3 ligase ITCH and DTX3L. Interacts with E3 ligase DTX3L; the interaction brings together STAM and HSG, promotes their recruitment to early endosomes and decreases STAM and HGS ubiquitination by ITCH. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct (By similarity). Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3 (By similarity). Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium (By similarity). Interacts with SNX1; the interaction is direct (By similarity). Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR (By similarity). Interacts with TRAK1. Interacts with TRAK2 (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity). Interacts with ARRDC3. Identified in a complex containing at least ARRDC4, AVPR2 and HGS. Interacts with LAPTM4B; promotes HGS ubiquitination.


Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

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