IL-2RA/CD25 Recombinant Rabbit mAb - #BF3725

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Product: IL-2RA/CD25 Recombinant Rabbit mAb
Catalog: BF3725
Description: Rabbit monoclonal antibody to IL-2RA/CD25
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 31kDa(Observed); 31kD(Calculated).
Uniprot: P01589

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 100ul $280 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm23172]
Specificity:
IL-2RA/CD25 Recombinant Rabbit mAb detects endogenous levels of IL-2RA/CD25.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Interleukin 2 receptor alpha chain; CD25; CD25 antigen; IDDM10; IL 2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2-RA; IL2R; IL2R, alpha chain; IL2RA; IL2RA_HUMAN; IMD41; Interleukin 2 receptor alpha; Interleukin 2 receptor; Interleukin-2 receptor subunit alpha; p55; t-cell growth factor receptor; TAC; TAC antigen; TCGFR;

Immunogens

Immunogen:

A synthetic peptide from human IL-2RA

Uniprot:

P01589(IL2RA_HUMAN) >>Visit HPA database.

Gene(ID):
IL2RA(3559)
Description:
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains,together with the common gamma chain (IL2RG),constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor,while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein,soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated,but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Sequence:
MDSYLLMWGLLTFIMVPGCQAELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQVAVAGCVFLLISVLLLSGLTWQRRQRKSRRTI

Research Backgrounds

Function:

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

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