JIP2 Antibody - #DF3260

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Product: JIP2 Antibody
Catalog: DF3260
Description: Rabbit polyclonal antibody to JIP2
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat, Zebrafish
Prediction: Pig, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 87 KD; 88kD(Calculated).
Uniprot: Q13387
RRID: AB_2835558

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Zebrafish
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(91%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
JIP2 Antibody detects endogenous levels of total JIP2.
RRID:
AB_2835558
Cite Format: Affinity Biosciences Cat# DF3260, RRID:AB_2835558.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C jun amino terminal kinase interacting protein 2; C-jun-amino-terminal kinase-interacting protein 2; Homologous to mouse JIP 1; IB 2; IB-2; IB2; Islet brain 2; Islet-brain-2; JIP 2; JIP-2; JIP2; JIP2_HUMAN; JNK interacting protein 2; JNK MAP kinase scaffold protein 2; JNK MAP kinase scaffold protein JIP2; JNK-interacting protein 2; MAPK8IP2; Mitogen activated protein kinase 8 interacting protein 2; Mitogen-activated protein kinase 8-interacting protein 2; PRKM8 interacting protein like; PRKM8IPL;

Immunogens

Immunogen:

A synthesized peptide derived from human JIP2, corresponding to a region within the internal amino acids.

Uniprot:

Q13387(JIP2_HUMAN) >>Visit HPA database.

Gene(ID):
MAPK8IP2(23542)
Expression:
Q13387 JIP2_HUMAN:

Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.

Sequence:
MADRAEMFSLSTFHSLSPPGCRPPQDISLEEFDDEDLSEITDDCGLGLSYDSDHCEKDSLSLGRSEQPHPICSFQDDFQEFEMIDDNEEEDDEDEEEEEEEEEGDGEGQEGGDPGSEAPAPGPLIPSPSVEEPHKHRPTTLRLTTLGAQDSLNNNGGFDLVRPASWQETALCSPAPEALRELPGPLPATDTGPGGAQSPVRPGCDCEGNRPAEPPAPGGTSPSSDPGIEADLRSRSSGGRGGRRSSQELSSPGSDSEDAGGARLGRMISSISETELELSSDGGSSSSGRSSHLTNSIEEASSPASEPEPPREPPRRPAFLPVGPDDTNSEYESGSESEPDLSEDADSPWLLSNLVSRMISEGSSPIRCPGQCLSPAPRPPGEPVSPAGGAAQDSQDPEAAAGPGGVELVDMETLCAPPPPAPAAPRPGPAQPGPCLFLSNPTRDTITPLWAAPGRAARPGRACSAACSEEEDEEDDEEEEDAEDSAGSPGGRGTGPSAPRDASLVYDAVKYTLVVDEHTQLELVSLRRCAGLGHDSEEDSGGEASEEEAGAALLGGGQVSGDTSPDSPDLTFSKKFLNVFVNSTSRSSSTESFGLFSCLVNGEEREQTHRAVFRFIPRHPDELELDVDDPVLVEAEEDDFWFRGFNMRTGERGVFPAFYAHAVPGPAKDLLGSKRSPCWVERFDVQFLGSVEVPCHQGNGILCAAMQKIATARKLTVHLRPPASCDLEISLRGVKLSLSGGGPEFQRCSHFFQMKNISFCGCHPRNSCYFGFITKHPLLSRFACHVFVSQESMRPVAQSVGRAFLEYYQEHLAYACPTEDIYLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Dog
91
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).

Subcellular Location:

Cytoplasm.
Note: Accumulates in cell surface projections.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.

Family&Domains:

Belongs to the JIP scaffold family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

References

1). CTRP3 alleviates cardiac ischemia/reperfusion injury via LAMP1/JIP2/JNK signaling pathway. Imaging, 2022 (PubMed: 35114641) [IF=0.4]

Application: WB    Species: Mice    Sample: myocardial tissue

Figure 7 Overexpression of CTRP3 ameliorated I/R injury. (A, B) The levels of LVEF and LVFS were detected by echocardiograph as indexes for cardiac function at the end of the 24 h reperfusion. (C) Representative images of stained myocardial tissues of mice in all groups. (D) The percentage of myocardial infarction area of all groups. (E, F) Cardiomyocyte apoptosis was determined by using TUNEL assay. (G–I) The production of ROS, MDA and cTn-I was determined. (J, K) Western blotting was performed to analyze the protein expression of CTRP3, LAMPA, JIP2, JNK and p-JNK in myocardial tissue.

Application: WB    Species: rat    Sample: H9C2 cells

Figure 6.| CTRP3 and LAMP1 overexpression accelerated activation of JIP2/JNK signaling pathway. (A) The relationship between CTRP3 and JIP2 was detected with Co‐IP.g.

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