RRM2 Recombinant Rabbit mAb - #BF4003

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Product: RRM2 Recombinant Rabbit mAb
Catalog: BF4003
Description: Rabbit monoclonal antibody to RRM2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Mouse
Mol.Wt.: 45 kDa(Observed); 45kD(Calculated).
Uniprot: P31350

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:3000, IHC 1:200-1:1000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm23450]
Specificity:
RRM2 Recombinant Rabbit mAb detects endogenous levels of RRM2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cb111; chunp6884; etID309896.20; R2; reductase M2 polypeptide variant 1; reductase M2 polypeptide variant 2; reductase M2 polypeptide variant 3a; reductase M2 polypeptide variant 3b; reductase M2 polypeptide variant 3c; reductase M2 polypeptide variant 3d; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase M2; Ribonucleotide reductase M2 polypeptide; Ribonucleotide reductase M2 subunit; ribonucleotide reductase protein r2 class I; Ribonucleotide reductase regulatory subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; Ribonucleotide reductase, R2 subunit; RIR2_HUMAN; RR2; RR2M; RRM2;

Immunogens

Immunogen:

A synthetic peptide from human RRM2

Uniprot:

P31350(RIR2_HUMAN) >>Visit HPA database.

Gene(ID):
RRM2(6241)
Description:
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters,which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
Sequence:
MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLASKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWESLKPEERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREFLFNAIETMPCVKKKADWALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVHKPSEERVREIIINAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLELGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSSPTENSFTLDADF

Research Backgrounds

Function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

PTMs:

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the ribonucleoside diphosphate reductase small chain family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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