POMC Recombinant Rabbit mAb - #BF4173

WB 1:1000, IHC 1:500-1:2000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Human, Mouse, Rat

Monoclonal [ReFirm23620]

POMC Recombinant Rabbit mAb detects endogenous levels of POMC.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

P01189(COLI_HUMAN) >>Visit HPA database.

P01189 COLI_HUMAN:

ACTH and MSH are produced by the pituitary gland.

This gene encodes a preproprotein that undergoes extensive,tissue-specific,post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and,depending on tissue type and the available convertases,processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used,adrenocorticotrophin,essential for normal steroidogenesis and the maintenance of normal adrenal weight,and lipotropin beta are the major end products. In other tissues,including the hypothalamus,placenta,and epithelium,all cleavage sites may be used,giving rise to peptides with roles in pain and energy homeostasis,melanocyte stimulation,and immune modulation. These include several distinct melanotropins,lipotropins,and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity,adrenal insufficiency,and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.

•    P01189 COLI_HUMAN:
• Protein BLAST With
• NCBI/
• ExPASy/
• Uniprot

MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRELTGQRLREGDGPDGPADDGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWGSPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE