Thymidine Kinase 1/TK1 Recombinant Rabbit mAb - #BF4268

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Product: Thymidine Kinase 1/TK1 Recombinant Rabbit mAb
Catalog: BF4268
Description: Rabbit monoclonal antibody to Thymidine Kinase 1/TK1
Application: WB IHC
Reactivity: Human
Mol.Wt.: 26 kDa(Observed); 25kD(Calculated).
Uniprot: P04183

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm23718]
Specificity:
Thymidine Kinase 1/TK1 Recombinant Rabbit mAb detects endogenous levels of Thymidine Kinase 1/TK1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2;

Immunogens

Immunogen:

A synthetic peptide from human Thymidine Kinase 1

Uniprot:

P04183(KITH_HUMAN) >>Visit HPA database.

Gene(ID):
TK1(7083)
Description:
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP,which is one component required for DNA replication. The encoded protein,whose levels fluctuate depending on the cell cycle stage,can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.
Sequence:
MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

Research Backgrounds

PTMs:

Phosphorylated on Ser-13 in mitosis.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the thymidine kinase family.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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