Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1473]
MSH2 antibody detects endogenous levels of total MSH2.
Cite Format: Affinity Biosciences Cat# BF0300, RRID:AB_2833498.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


BAT26; COCA 1; COCA1; DNA mismatch repair protein Msh2; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2;



Purified recombinant fragment of human MSH2 expressed in E. Coli.

P43246 MSH2_HUMAN:

Ubiquitously expressed.

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

PTMs - P43246 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K65 Ubiquitination
Y66 Phosphorylation
K73 Acetylation
K73 Ubiquitination
S77 Phosphorylation
K104 Acetylation
K104 Methylation
K113 Acetylation
K172 Ubiquitination
T206 Phosphorylation
K212 Ubiquitination
R219 Methylation
K235 Ubiquitination
Y238 Phosphorylation
K249 Ubiquitination
K332 Ubiquitination
K347 Ubiquitination
K430 Sumoylation
S479 Phosphorylation
K491 Ubiquitination
K509 Ubiquitination
K531 Ubiquitination
K537 Ubiquitination
K550 Acetylation
K555 Acetylation
K555 Ubiquitination
S558 Phosphorylation
Y563 Phosphorylation
K565 Ubiquitination
K567 Ubiquitination
Y570 Phosphorylation
K635 Acetylation
K635 Ubiquitination
S717 Phosphorylation
K720 Ubiquitination
S738 Phosphorylation
T740 Phosphorylation
K741 Ubiquitination
S743 Phosphorylation
K845 Ubiquitination
S860 Phosphorylation
Y863 Phosphorylation
K871 Ubiquitination
K872 Ubiquitination
K882 Ubiquitination
K892 Ubiquitination
T897 Phosphorylation
S900 Phosphorylation
K912 Ubiquitination
K918 Ubiquitination
S921 Phosphorylation

Research Backgrounds


Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.


Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2-MSH3 (MutS beta). Both heterodimers form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9; the interaction recruits MCM9 to chromatin. Interacts with MCM8. Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1.


Belongs to the DNA mismatch repair MutS family.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

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