TPM1 Recombinant Rabbit mAb - #BF4526

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Product: TPM1 Recombinant Rabbit mAb
Catalog: BF4526
Description: Rabbit monoclonal antibody to TPM1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 33 kDa(Observed); 33kD(Calculated).
Uniprot: P09493

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IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC: 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23976]
Specificity:
TPM1 Recombinant Rabbit mAb detects endogenous levels of TPM1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA986836; AI854628; Alpha tropomyosin; alpha-TM; Alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; HTM alpha; HTM-alpha; OTTHUMP00000163688; sarcomeric tropomyosin kappa; TM2; Tmpa; TMSA; Tpm-1; TPM1; TPM1_HUMAN; tropomyosin 1 (alpha); tropomyosin 1 (alpha) isoform 1; tropomyosin 1 (alpha) isoform 2; tropomyosin 1 (alpha) isoform 3; tropomyosin 1 (alpha) isoform 4; tropomyosin 1 (alpha) isoform 5; tropomyosin 1 (alpha) isoform 6; tropomyosin 1 (alpha) isoform 7; Tropomyosin 1; Tropomyosin alpha 1 chain; Tropomyosin alpha-1 chain; Tropomyosin, skeletal muscle alpha; Tropomyosin-1;

Immunogens

Immunogen:

A synthetic peptide from human TPM1

Uniprot:

P09493(TPM1_HUMAN) >>Visit HPA database.

Gene(ID):
TPM1(7168)
Expression:
P09493 TPM1_HUMAN:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Description:
This gene is a member of the tropomyosin family of highly conserved,widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle,where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells,alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
Sequence:
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Research Backgrounds

Function:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

PTMs:

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Associates with F-actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Family&Domains:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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