CTLA4 Recombinant Rabbit mAb - #BF4618

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Product: CTLA4 Recombinant Rabbit mAb
Catalog: BF4618
Description: Rabbit monoclonal antibody to CTLA4
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 25 kDa(Observed); 25kD(Calculated).
Uniprot: P16410

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IHC 1:200-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse
Clonality:
Monoclonal [ReFirm24068]
Specificity:
CTLA4 Recombinant Rabbit mAb detects endogenous levels of CTLA4.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALPS5; CD 152; CD; CD152; CD152 antigen; CD152 isoform; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; CTLA4; CTLA4_HUMAN; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte antigen 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated 4, soluble isoform, included; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated antigen 4 short spliced form; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; GRD4; GSE; ICOS; IDDM12; insulin-dependent diabetes mellitus 12; Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; OTTHUMP00000216623;

Immunogens

Immunogen:

A synthetic peptide from human CTLA4

Uniprot:

P16410(CTLA4_HUMAN) >>Visit HPA database.

Gene(ID):
CTLA4(1493)
Expression:
P16410 CTLA4_HUMAN:

Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.

Description:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain,a transmembrane domain,and a cytoplasmic tail. Alternate transcriptional splice variants,encoding different isoforms,have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond,while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus,Graves disease,Hashimoto thyroiditis,celiac disease,systemic lupus erythematosus,thyroid-associated orbitopathy,and other autoimmune diseases.
Sequence:
MACLGFQRHKAQLNLATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDFLLWILAAVSSGLFFYSFLLTAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQFQPYFIPIN

Research Backgrounds

Function:

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

PTMs:

N-glycosylation is important for dimerization.

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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For Research Use Only.
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