Product: ARFGAP3 Antibody
Catalog: DF3748
Description: Rabbit polyclonal antibody to ARFGAP3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 57kd,66kDa; 57kD(Calculated).
Uniprot: Q9NP61
RRID: AB_2836112

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Zebrafish(80%), Bovine(90%), Horse(90%), Sheep(90%), Dog(90%), Chicken(90%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
ARFGAP3 Antibody detects endogenous levels of total ARFGAP3.
RRID:
AB_2836112
Cite Format: Affinity Biosciences Cat# DF3748, RRID:AB_2836112.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor GTPase activating protein 1; ADP ribosylation factor GTPase activating protein 3; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFG3_HUMAN; ArfGAP 3; ARFGAP1; ARFGAP3; FLJ45618;

Immunogens

Immunogen:

A synthesized peptide derived from human ARFGAP3, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q9NP61 ARFG3_HUMAN:

Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.

Sequence:
MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSNWSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVPPLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPVETTLENNEGGQEQGPSVEGLNVPTKATLEVSSIIKKKPNQAKKGLGAKKGSLGAQKLANTCFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYKDLEIQMKKDEKMNISGKKNVDSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSDDSYFTSSSSYFDEPVELRSSSFSSWDDSSDSYWKKETSKDTETVLKTTGYSDRPTARRKPDYEPVENTDEAQKKFGNVKAISSDMYFGRQSQADYETRARLERLSASSSISSADLFEEPRKQPAGNYSLSSVLPNAPDMAQFKQGVRSVAGKLSVFANGVVTSIQDRYGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Chicken
90
Xenopus
80
Zebrafish
80
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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