Smad9 Antibody - #AF5114

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Product: Smad9 Antibody
Catalog: AF5114
Description: Rabbit polyclonal antibody to Smad9
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Sheep, Chicken
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: O15198
RRID: AB_2837600

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
Smad9 Antibody detects endogenous levels of total Smad9.
RRID:
AB_2837600
Cite Format: Affinity Biosciences Cat# AF5114, RRID:AB_2837600.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MAD homolog 9; Madh6; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD, mothers against DPP homolog 9 (Drosophila); SMAD8A; SMAD8B; Smad9; SMAD9_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Smad9, corresponding to a region within the internal amino acids.

Uniprot:

O15198(SMAD9_HUMAN) >>Visit HPA database.

Gene(ID):
SMAD9(4093)
Expression:
O15198 SMAD9_HUMAN:

Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.

Description:
SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.
Sequence:
MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYPHSPGSPSEPESPYQHSVDTPPLPYHATEASETQSGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Chicken
86
Horse
75
Dog
70
Rabbit
67
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).

PTMs:

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.

Subcellular Location:

Cytoplasm. Nucleus.
Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.

Family&Domains:

Belongs to the dwarfin/SMAD family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

References

1). Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy. Journal of nanobiotechnology, 2025 (PubMed: 41139778) [IF=10.2]

Application: WB    Species: Rat    Sample:

Fig. 6 (a) Cluster analysis of total differentially expressed genes among the Control, OGD/R, and OGD/R + LDH groups, P 0.58, n = 3. (b) Volcano plot analysis of total differentially expressed genes between Control and OGD/R groups, P 0.58. (c) Volcano plot analysis of total differentially expressed genes between OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (d) KEGG enrichment analysis of total differentially expressed genes between Control and OGD/R groups, P 1, n = 3. (e) KEGG enrichment analysis of total differentially expressed genes between OGD/R and OGD/R + LDH groups, P 1, n = 3. (f) Representative western blot bands of p-PI3K/PI3K/p-AKT/AKT/TGF-β/Smad9/β-actin after different treatments. (g) Quantification of p-PI3K/PI3K and p-AKT/AKT protein levels from (f), n = 4. (h) Co-staining images of p-PI3K9 (green) and α-actinin (red) after different treatments on Day 14, Scale bar = 20 μm, n = 5. (i, j) Heat map of expressed genes related to apoptosis signaling pathway and sprouting angiogenesis among the Control, OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (k) GSEA analysis of sprouting angiogenesis between OGD/R and OGD/R + LDH groups, P 1.0, n = 3. (l) GSEA analysis of TGF-β signaling pathway between OGD/R and OGD/R + LDH groups, P 1.0, n = 3. (m) Heat map of expressed genes related to TGF-β signaling pathway among the Control, OGD/R and OGD/R + LDH groups, P 0.58, n = 3. (n) Quantification of TGF-β2 and Smad9 protein levels from (f), n = 4. Data are presented as mean values ± SEM
2). Dose-optimized HILT promotes peripheral nerve repair through BMP4-Smad9-mediated inhibition of neuroinflammation and oxidative stress. APL bioengineering, 2026 (PubMed: 41799557) [IF=6.6]

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