Product: MMP9 Antibody
Catalog: AF5228
Source: Rabbit
Application: WB, IHC, IF/ICC, IHC-P, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse
Mol.Wt.: 78 kD(active),92-105kD(Pro mmp9); 78kD(Calculated).
Uniprot: P14780
RRID: AB_2837714

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

IF/ICC 1:200, IHC-p 1:50-1:200, WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(82%), Horse(82%)
MMP9 Antibody detects endogenous levels of total MMP9.
Cite Format: Affinity Biosciences Cat# AF5228, RRID:AB_2837714.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; CLG 4B; CLG4B; Collagenase Type 4 beta; Collagenase type IV 92 KD; EC; Gelatinase 92 KD; Gelatinase B; Gelatinase beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; MMP 9; MMP-9; MMP9; MMP9_HUMAN; Type V collagenase;


P14780 MMP9_HUMAN:

Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes.

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14780 As Substrate

Site PTM Type Enzyme
T37 Phosphorylation
C99 S-Nitrosylation
Y241 Phosphorylation
S628 Phosphorylation

Research Backgrounds


May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.


Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.

N- and O-glycosylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.

Subunit Structure:

Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with LCN2. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1.

(Microbial infection) Interacts with Staphylococcus aureus protein SSL5; this interaction inhibits MMP9 activity.


The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.


1). Liao Y et al. CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation. Cell Death Differ 2021 Apr;28(4):1347-1363. (PubMed: 33162555) [IF=12.067]

Application: WB    Species: Human    Sample: thyroid cancer cells

Fig. 3 Regulation of CRSP8 on migration, invasion, and apoptosis of thyroid cancer cells. A Cell migration and B cell invasion were analyzed in thyroid cancer cells transfected with CRSP8 specific siRNAs or its overexpression plasmids, and the relative rate of migration was calculated, the number of invading cells was counted. C Western blot analysis of the expression of MMP-9, EMT markers (E-cadherin, N-cadherin, β-catenin, Vimentin) in THJ-29T and FTC-133 cells following CRSP8 silencing or overexpression. THJ-21T and THJ-29T cells were treated with CRSP8 specific siRNAs for 48 h, then D FACS analysis and E acridine orange/ethidium bromide fluorescence staining were performed, and the apoptotic cell ratios were counted respectively. F Western blot analysis of the expression of Bcl-2, Bad, Bim, Bax, cleaved caspase-3, and cleaved caspase-9 proteins in thyroid cancer cells following CRSP8 knockdown. The data represent the mean ± SD of at least three independent experiments (four independent experiments in 3D), and the level of significance was indicated by ***P < 0.001.

2). Ai XY et al. Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action. Theranostics 2017 Jan 5;7(2):425-435 (PubMed: 28255340) [IF=11.600]

Application: WB    Species: human    Sample:

Figure 6. PnAg regulates gp130/Jak/Stat3 signaling pathway (A) and (B) NIH-3T3 and HaCat Cells were treated with PnAg at different concentrations and cell viability was tested using MTT analysis. (C) Wound healing assay reflected the effect of PnAg on cell migration. (D) Binding mode of PnAg in the active pocket of gp130. (E) and (F) MMPs activity and expression levels of Stat3, VEGF, TGFB-1, and TGFB1 detected using zymographic and Western blot assays. (G) Diagram of the proposed function of PnAg in wound inflammation and re-epithelialization controls.

Application: IHC    Species: human    Sample:

Figure 2. PnAg promotes wound healing in SD rats. (A) Photographs of rat skin full-thickness excision wounds on different post-excision days. (B) Change in wound areas of SD rats after treatment; (C) and (D) Expression levels of collagen I, NF-κB, TGF-ß, MMP-2, and MMP-9 in tissues on day 7 and 17 detected by immunohistochemistry. (E) Histogram of protein expression levels in these tissues. (F) and (G) Histomorphological changes in wound tissues stained by Masson trichrome and HE on day 17.

3). Yang Y et al. The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming. Cancer Lett 2019 Apr 4 (PubMed: 30954648) [IF=9.756]

4). Luo Y et al. Foxq1 promotes metastasis of nasopharyngeal carcinoma by inducing vasculogenic mimicry via the EGFR signaling pathway. Cell Death Dis 2021 Apr 19;12(5):411. (PubMed: 33875643) [IF=9.685]

Application: IF/ICC    Species: mouse    Sample: 5–8F cells and CNE1

Fig. 4| Foxq1 promotes the EGFR signaling pathway and VM-related genes through a direct binding to the EGFR promoter. A Immunofluorescence staining of Foxq1,EGFR,VE-cadherin,MMP2, and MMP9 in Foxq1 dysregulated 5–8F, and CNE1; Scale bars represent 50 μm. qRTPCR (above) and western blot (down) were used to monitor the expression of EGFR signaling pathway and VM-related genes in 5–8F, and CNE1 after Foxq1 downregulation (B, C) or overexpression (D, E)

Application: WB    Species: mouse    Sample: 5–8F cells

Fig. 6 |Erlotinib and Nimotuzumab could inhibit Foxq1-induced VM formation and NPC growth and metastasis in vivo.F Statistical results of metastatic nodules of each group; p < 0.001. The expression of related genes in xenograft tumors from each group were monitored by qRT-PCR (G) and western blot (H).

5). Han X et al. Stimulation of α7 Nicotinic Acetylcholine Receptor by Nicotine Suppresses Decidual M1 Macrophage Polarization Against Inflammation in Lipopolysaccharide-Induced Preeclampsia-Like Mouse Model. Front Immunol 2021 Apr 28;12:642071. (PubMed: 33995360) [IF=8.786]

Application: IF/ICC    Species: Mice    Sample:

Figure 4 Nicotine up-regulated the decreased MMP-9 expression in placentas from LPS-induced PE-like mice. Representative immunofluorescent images (A) and graph bar (B) showed changes in levels of MMP-9 immunoreactivity (green) in the trophoblast layer of placentas from all animal groups. Scale bar=50 μm. *P < 0.05 and **P < 0.01.

6). Meng J et al. Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma. Oncogene 2018 Aug 7 (PubMed: 30087438) [IF=8.756]

Application: IHC    Species: human    Sample: HCC cells

Fig. 1| Hsp90βassociates with vasculogenic mimicry and poor prog-nosis in HCC. e Representative images of Hsp90β, Hsp90α, VE-cadherin,E-cadherin, Vimentin, MMP2, and MMP9 expression in VM/Hsp90βnegative and positive HCC tissue samples. All images are repre-sentative.

Application: WB    Species: human    Sample: PLC-PRF-5 cells

Fig. 5| Hsp90β promotes Twist1 nuclear translocation and binding to VE-cadherin promoter to increase VM-related gene networks. eWestern blot analysis of VM and EMT-related markers, including VE-cadherin, VEGFR1, VEGFR2, E-cadherin, Vimentin, MMP2, and MMP9 in PLC-PRF-5 cells overexpressed Hsp90β or under lack of Twist1.

7). Duan R et al. A De Novo Frameshift Mutation in TNFAIP3 Impairs A20 Deubiquitination Function to Cause Neuropsychiatric Systemic Lupus Erythematosus. J Clin Immunol 2019 Oct 17 (PubMed: 31625129) [IF=8.542]

8). Cai Y et al. Dynamic change of MMP-9 in the diabetic stroke visualized by optical imaging and treated with CD28 superagonist. Biomater Sci 2021 Feb 15. (PubMed: 33585848) [IF=7.590]

Application: WB    Species: Mice    Sample: diabetic mice

Fig. 4 CD28 SA treatment reduced the expression of MMP-9 in STZ-induced diabetic stroke on day 7 post stroke. (A) Representative immunofluorescence images and (B) quantification of MMP-9 in the ischemic brain of diabetic mice on day 7 post stroke. The expression of MMP-9 was significantly downregulated by CD28 SA administration on day 7 post stroke (n = 7–8). (C) Western blotting and (D) quantitative analysis showed that the expression of MMP-9 was significantly decreased after CD28 treatment in diabetic mice on day 7 post stroke (n = 3–4). (E) The TBR values of NIRF images were significantly correlated with the fluorescence integrated optical density (IOD) of MMP-9 on immunofluorescent staining, indicating the high sensitivity of the probe for in vivo monitoring of the expression of MMP-9. Mean ± S.E.M. *p < 0.05.

9). Song S et al. A novel long noncoding RNA, TMEM92‐AS1, promotes gastric cancer progression by binding to YBX1 to mediate CCL5. Mol Oncol 2021 Apr;15(4):1256-1273. (PubMed: 33247987) [IF=7.449]

Application: WB    Species: human    Sample: BGC823 cells

Figure 3 TMEM92-AS1 regulate expression of cellular functional genes. (A and B) RT-qPCR and western blot assays were used to detect expression changes of cellular functional genes and proteins. (

Application: WB    Species: human    Sample: BGC823 and SGC7901 cells

Figure 4(F) RT-qPCR and western blot were used to detect changes in TMEM92-AS1-related functional genes expression after knockdown of CCL5. *P<0.05, **P<0.005 by Student’s t-test. For all of these experiments, values were mean±SD (error bars) of triplicate samples in representative experiments.

10). Wang P et al. Selenium Attenuates TBHP-Induced Apoptosis of Nucleus Pulposus Cells by Suppressing Mitochondrial Fission through Activating Nuclear Factor Erythroid 2-Related Factor 2. Oxid Med Cell Longev 2022 Apr 11;2022:7531788. (PubMed: 35450408) [IF=7.310]

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