HDAC10 Antibody - #AF5474

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Product: HDAC10 Antibody
Catalog: AF5474
Description: Rabbit polyclonal antibody to HDAC10
Application: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Dog
Mol.Wt.: 71 kDa; 71kD(Calculated).
Uniprot: Q969S8
RRID: AB_2837956

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(85%), Dog(92%)
Clonality:
Polyclonal
Specificity:
HDAC10 Antibody detects endogenous levels of total HDAC10.
RRID:
AB_2837956
Cite Format: Affinity Biosciences Cat# AF5474, RRID:AB_2837956.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZP761B039; HD 10; HD10; HDA10_HUMAN; HDAC 10; Hdac10; Histone deacetylase 10; MGC149722; OTTHUMP00000028555;

Immunogens

Immunogen:

A synthesized peptide derived from human HDAC10, corresponding to a region within N-terminal amino acids.

Uniprot:

Q969S8(HDA10_HUMAN) >>Visit HPA database.

Gene(ID):
HDAC10(83933)
Expression:
Q969S8 HDA10_HUMAN:

Widely expressed with high levels in liver and kidney.

Description:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Sequence:
MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Pig
85
Horse
77
Bovine
77
Sheep
77
Rabbit
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Excluded from nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with high levels in liver and kidney.

Family&Domains:

Belongs to the histone deacetylase family. HD type 2 subfamily.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). Myeloid-specific Hdac10 deletion protects against LPS-induced acute lung injury via P62 acetylation at lysine 165. Respiratory research, 2024 (PubMed: 38956592) [IF=4.7]

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 1 HDAC10 expression is increased in the lung macrophages following LPS exposure. A HDAC10 mRNA level in LPS-exposed ALI mice lung tissues. B, C HDAC10 protein level in LPS-stimulated ALI mice lung tissues. D, E Increased HDAC10 level in lung tissues of LPS-stimulated ALI mice measured by IHC staining for HDAC10. F, G IF staining of HDAC10 and F4/80 in mice lung tissues before or after LPS exposure. H, I WB analysis of HDAC10 in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. J, K HDAC10 mRNA expression in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. The data are expressed as the mean ±SEM (n = 3-5 mice/group). The data of Fig. 1G expressed as the median. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are representative of three independent experiments with similar results (A, E, J, K). See also Fig. S1

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 1 HDAC10 expression is increased in the lung macrophages following LPS exposure. A HDAC10 mRNA level in LPS-exposed ALI mice lung tissues. B, C HDAC10 protein level in LPS-stimulated ALI mice lung tissues. D, E Increased HDAC10 level in lung tissues of LPS-stimulated ALI mice measured by IHC staining for HDAC10. F, G IF staining of HDAC10 and F4/80 in mice lung tissues before or after LPS exposure. H, I WB analysis of HDAC10 in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. J, K HDAC10 mRNA expression in MH-S cells after 100 μg/ml LPS stimulation for 0-12 h and at different concentrations for 12 h. The data are expressed as the mean ±SEM (n = 3-5 mice/group). The data of Fig. 1G expressed as the median. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are representative of three independent experiments with similar results (A, E, J, K). See also Fig. S1

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