NTF3 Antibody - #DF6105

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Product: NTF3 Antibody
Catalog: DF6105
Description: Rabbit polyclonal antibody to NTF3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29kDa; 29kD(Calculated).
Uniprot: P20783
RRID: AB_2838073

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NTF3 Antibody detects endogenous levels of total NTF3.
RRID:
AB_2838073
Cite Format: Affinity Biosciences Cat# DF6105, RRID:AB_2838073.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 precursor; Neurotrophin-3; Neurotrophin3; NGF 2; NGF-2; NGF2; NT 3; NT-3; NT3; NTF 3; NTF3; NTF3_HUMAN;

Immunogens

Immunogen:
Uniprot:

P20783(NTF3_HUMAN) >>Visit HPA database.

Gene(ID):
NTF3(4908)
Expression:
P20783 NTF3_HUMAN:

Brain and peripheral tissues.

Description:
NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4 (1). NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes (1-3). NT-3 is required for the development of peripheral sensory neurons (4). NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB (1).
Sequence:
MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20783 As Substrate

Site PTM Type Enzyme
Y59 Phosphorylation
Y99 Phosphorylation
S101 Phosphorylation
Y123 Phosphorylation
T222 Phosphorylation

Research Backgrounds

Function:

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain and peripheral tissues.

Family&Domains:

Belongs to the NGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

References

1). Electroacupuncture improves neuronal plasticity through the A2AR/cAMP/PKA signaling pathway in SNL rats. Neurochemistry International (PubMed: 33577869) [IF=4.2]

Application: WB    Species: rat    Sample: spinal cord

Fig. 8. EA decreased the protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in SNL rats, while SCH58261 reversed this effect. (A), (B), (C), (D), (E) Protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in the spinal cord and quantification data of the expression of NTF3/GAPDH, NTF4/ GAPDH, Synaptophysin/GAPDH and Synapsin I/GAPDH in each group. n = 5. Columns represent the mean ± SD. $$p < 0.01 vs. the S group; #p < 0.05, ##p < 0.01 vs. the M group; &&p < 0.01 vs. the M + EA group.
2). Oxycodone regulates incision-induced activation of neurotrophic factors and receptors in an acute post-surgery pain rat model. Journal of Pain Research (PubMed: 30464584) [IF=2.7]

Application: WB    Species: rat    Sample: spinal cord

Figure 3 |The altered expression of NTs in the spinal cord 24 hours after PI and the effects of oxycodone on NTs.Notes: (A, C, and E) The mRNA level of NGF, BDNF, and NT-3. (B, D, and F) The upper panels represent results of the Western blots. The lower panels show the semiquantitative analysis of NGF, BDNF, and NT-3 after normalization to the corresponding β-actin (*P<0.05, **P<0.01, ***P<0.001 vs control group; #P<0.05, ##P<0.01, ###P<0.001 vs PI, one-way ANOVA followed by Bonferroni’s multiple comparison post hoc test, n=3–4 rats per group). Each value represents mean±SEM. PI+OXY, postoperative oxycodone group; OXY+PI, preoperative oxycodone group.
3). Volatile Oil from Acorus gramineus Ameliorates the Injury Neurons in the Hippocampus of Amyloid Beta 1-42 Injected Mice. ANATOMICAL RECORD (PubMed: 31443117)

Application: IHC    Species: mouse    Sample: hippocampus

Fig. 7. |The immunohistochemical observation of NT3 expression in the hippocampus of mice among all experimental groups. (A-A00) Control group, (B-B00) saline group, (C-C00) AD group; (D-D00) AD plus per 10 g body weight 2.5 μL volatile oil group, (E-E00) AD plus per 10 g body weight 5 μL volatile oil group. Scale bars = 50 μm in image A–E, 20 μm in A0-E0, 10 μm in A00-E00.

Application: WB    Species: mouse    Sample: hippocampus

Fig. 7. |The immunohistochemical observation of NT3 expression in the hippocampus of mice among all experimental groups. The western blot results showed the expression levels of NT3 protein (F). The histogram was plotted according to the comparison of the relative density for NT3 in all groups (G). *P < 0.05 AD group vs. control group; #P < 0.05 different doses of volatile oil groups vs. AD group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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