STEA3 Antibody | Affinity Biosciences

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STEA3 Antibody - Western blot analysis of Mouse liver tissue lysates, using STEAP3 Antibody.

Product:	STEA3 Antibody
Catalog:	DF6122
Description:	Rabbit polyclonal antibody to STEA3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	55kDa; 55kD(Calculated).
Uniprot:	Q658P3
RRID:	AB_2838089

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

STEA3 Antibody detects endogenous levels of total STEA3.

RRID:

AB_2838089
Cite Format: Affinity Biosciences Cat# DF6122, RRID:AB_2838089.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1010001D01Rik; Dudlin 2; Dudlin2; Dudulin 2; Dudulin-2; Dudulin2; hpHyde; hTSAP6; Metalloreductase STEAP3; MGC93147; pHyde; Six transmembrane epithelial antigen of prostate 3; Six transmembrane prostate protein 3; Six-transmembrane epithelial antigen of prostate 3; STEA 3; STEA3_HUMAN; STEAP 3; STEAP family member 3; STEAP family member 3 metalloreductase; STEAP3; STMP 3; STMP3; TSAP 6; TSAP6; Tumor suppressor activated pathway protein 6; Tumor suppressor pHyde; Tumor suppressor-activated pathway protein 6;

Immunogens

Immunogen:

Uniprot:

Q658P3(STEA3_HUMAN) >>Visit HPA database.

Gene(ID):

STEAP3(55240)

Expression:

Q658P3 STEA3_HUMAN:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Description:

Caspases modulate apoptosis in various ways. Specifically, Caspase-3, a death protease, is instrumental in cleaving cellular proteins, dismantling the cell and forming apoptotic bodies. pHyde has a potential role as a tumor suppressor by inducing caspase-3-mediated apoptosis and stimulating p53 expression. A dose-dependent increase in caspase-3 activity is observed in transduced pHyde DU145 cells. Furthermore, caspase-3 may be necessary for pHyde-mediated apoptosis. The pHyde gene may upregulate the apoptosis pathway and thus have a potential application in cancer gene therapy. Recombinant pHyde inhibits the growth of human prostate cancer cell lines DU145 and LNCaP in vitro. DU145 tumors may be reduced significantly in vivo when nude mice are injected with recombinant pHyde. pHyde also has a demonstrated growth inhibitory effect on human breast cancer cells. This suggests that pHyde may have a role in inhibiting different tumor types.

Sequence:

Q658P3 STEA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAMPLRLLPAWKVPTLLALGLFVCFYAYNFVRDVLQPYVQESQNKFFKLPVSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRAHRYDLVNLAVKQVLANKSHLWVEEEVWRMEIYLSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSSLGFVALVLSTLHTLTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILAKALFLLPCISRRLARIRRGWERESTIKFTLPTDHALAEKTSHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q658P3 As Substrate

Q658P3

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
S11	Phosphorylation	Uniprot
S17	Phosphorylation	Uniprot
S19	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
K23	Ubiquitination	Uniprot
K30	Ubiquitination	Uniprot
S42	Phosphorylation	Uniprot
T141	Phosphorylation	Uniprot
Y239	Phosphorylation	Uniprot
S243	Phosphorylation	Uniprot
N256	N-Glycosylation	Uniprot
Y288	Phosphorylation	Uniprot
Y331	Phosphorylation	Uniprot
K339	Ubiquitination	Uniprot
N344	N-Glycosylation	Uniprot
S469	Phosphorylation	Uniprot
T470	Phosphorylation	Uniprot
K472	Ubiquitination	Uniprot
T474	Phosphorylation	Uniprot
T477	Phosphorylation	Uniprot
K484	Ubiquitination	Uniprot
S486	Phosphorylation	Uniprot

Research Backgrounds

Q658P3_STEA3_HUMAN

Function:

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

PTMs:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.

Glycosylated.

Subcellular Location:

Endosome membrane>Multi-pass membrane protein.
Note: Localizes to vesicular-like structures at the plasma membrane and around the nucleus.

Tissue Specificity:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Subunit Structure:

Homodimer. Interacts with BNIP3L, MYT1, RHBDL4/RHBDD1 and TCTP.

Family&Domains:

Belongs to the STEAP family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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STEA3 Antibody - #DF6122