ATG7 Antibody - #DF6130

FIGURE 4|Effect of calycosin on autophagy in muscle of 5/6 Nx rats. A, Representative images of autophagosomes observed in muscle of rat using TEM. B-E, Representative images and scores of LC3A/B and ATG7 expression in muscle of rats using IHC staining (×200). Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles. A-B,Representative images and scores of the expression of CARM1 and H3R17me2a in muscle sections using IHC staining (×200).Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group. C-E, Representative immune blots of key proteins involved in muscle atrophy and proteins associated with the AMPK/SKP2/CARM1 signalling pathway

FIGURE 5 DEX activated the AMPKα/BECN1 and ATG5/ATG7/NCOA4 signalling pathways via GRα. (A) Representative western blot results of placental tissues. (B) Representative western blot results showing HTR8/SVeno and TEV-1 expression. Data shown in the bar chart are presented as mean ± SD. Statistical analysis between two groups in (A) was performed by the student t-test, inter-group statistical analysis in (B) was performed by one-way ANOVA. ** in (A) represent p  0.05, and ** represent p 

Figure 10. Kunkui Baoshen decoction modulates ferroptosis by regulating nuclear receptor coactivator 4-mediated ferritinophagy. A-G: Western blot and quantitative reverse transcription-polymerase chain reaction analyses of autophagy related 7 (ATG7), nuclear receptor coactivator 4 (NCOA4), microtubule-associated protein light chain 3 (LC3), sequestosome 1 (SQSTM1/p62), and ferritin heavy chain 1 (FTH1) in normal and high glucose groups; H: Co-localization of ferritinophagy-related proteins observed by immunofluorescence double staining in human kidney-2 cells (scale bars = 50 μm); I-O: Western blot and quantitative reverse transcription-polymerase chain reaction analyses of ATG7, NCOA4, LC3, SQSTM1/p62, and FTH1 in the four groups; P-V: Western blot analysis of ATG7, glutathione peroxidase 4, solute carrier family 7 member 11, LC3, SQSTM1/p62, and FTH1 after NCOA4 knockdown. NG: Normal group; HG: High glucose; ATG7: Autophagy related 7; NCOA4: Nuclear receptor coactivator 4; LC3: Microtubule-associated protein light chain 3; SQSTM1/p62: Sequestosome 1; FTH1: Ferritin heavy chain 1; Fer-1: Ferrostatin-1; KKBS:: Kunkui Baoshen decoction; GPX4: Glutathione peroxidase 4; SLC7A11: Solute carrier family 7 member 11; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; si-NC: Negative control small interfering RNA; si-NCOA4: Nuclear receptor coactivator 4-targeting small interfering RNA. aP < 0.05 vs normal group, bP < 0.01 vs normal group, dP < 0.0001 vs normal group; eP < 0.05 vs high glucose group, fP < 0.01 vs high glucose group, gP < 0.001 vs high glucose group, hP < 0.0001 vs high glucose group; iP < 0.05 vs high glucose + ferrostatin-1 group, jP < 0.01 vs high glucose + ferrostatin-1 group, kP < 0.001 vs high glucose + ferrostatin-1 group, lP < 0.0001 vs high glucose + ferrostatin-1 group; mP < 0.05 vs negative control small interfering RNA group; nP < 0.05 vs nuclear receptor coactivator 4-targeting small interfering RNA group, and oP < 0.01 vs nuclear receptor coactivator 4-targeting small interfering RNA group.

Figure 5. |Inhibition of autophagy with specific shRNA targeting ATG5, ATG7 or BECN1 reduces RSV replication. (A-C) Hep2 cells stably expressing specific shRNA against ATG5 (Hep2-sh-ATG5), ATG7 (Hep2-sh-ATG7), BECN1 (Hep2-sh-BECN1) or luciferase (Hep2-sh-luciferase) were established as described in Materials and Methods. The silencing efficiency of ATG5 shRNA (A), ATG7 shRNA (B) and BECN1 shRNA (C) were validated by immunoblotting with anti-ATG5, anti-ATG7, anti-BECN1 and anti-GAPDH antibodies respectively.

Fig. 4. Effects of AOS on enhancing the lipophagy and free fatty acid (FFA) β-oxidation level in the NAFLD mouse livers. (A) the mRNA expression in the lipophagy and FFA β-oxidation pathways, (B) western blot results of lipophagy pathway, (C) the protein levels of lipophagy pathway, (D) western blot results of FFA β-oxidation pathway, (E) the protein levels of FFA β-oxidation pathway. The data are shown as the mean ± SEM. Values with different letters represent significant differences (p < 0.05)..