ILK Antibody - #DF6141

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Product: ILK Antibody
Catalog: DF6141
Description: Rabbit polyclonal antibody to ILK
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 59kDa; 51kD(Calculated).
Uniprot: Q13418
RRID: AB_2838108

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
ILK Antibody detects endogenous levels of total ILK.
RRID:
AB_2838108
Cite Format: Affinity Biosciences Cat# DF6141, RRID:AB_2838108.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; DKFZp686F1765; Epididymis secretory protein Li 28; HEL S 28; ILK 1; ILK 2; ILK; ILK-1; ILK-2; ILK_HUMAN; ILK1; ILK2; Integrin linked kinase 2; Integrin linked Kinase; Integrin linked protein kinase; Integrin-linked protein kinase; p59; p59ILK;

Immunogens

Immunogen:
Uniprot:

Q13418(ILK_HUMAN) >>Visit HPA database.

Gene(ID):
ILK(3611)
Expression:
Q13418 ILK_HUMAN:

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Description:
Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).
Sequence:
MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13418 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T69 Phosphorylation
S76 Phosphorylation
K85 Ubiquitination
K139 Ubiquitination
K154 Ubiquitination
K165 Ubiquitination
K170 Methylation
K170 Ubiquitination
T172 Phosphorylation
T173 Phosphorylation P17252 (PRKCA) , Q13153 (PAK1)
T181 Phosphorylation P17252 (PRKCA)
K184 Ubiquitination
S186 Phosphorylation
K191 Sumoylation
K191 Ubiquitination
K198 Ubiquitination
S204 Phosphorylation
K209 Ubiquitination
K220 Ubiquitination
S232 Phosphorylation
S246 Phosphorylation Q13153 (PAK1)
K341 Ubiquitination
S343 Phosphorylation Q13418 (ILK)
C346 S-Nitrosylation
K423 Ubiquitination
K426 Acetylation
K426 Ubiquitination
K435 Ubiquitination
K438 Ubiquitination
K448 Ubiquitination

PTMs - Q13418 As Enzyme

Substrate Site Source
E9PAV3 (NACA) S1906 Uniprot
O14950 (MYL12B) T19 Uniprot
O14950 (MYL12B) S20 Uniprot
O14974-1 (PPP1R12A) T696 Uniprot
O14974 (PPP1R12A) T710 Uniprot
P23528 (CFL1) S3 Uniprot
P31749 (AKT1) S473 Uniprot
Q13418-1 (ILK) S343 Uniprot
Q13765 (NACA) S43 Uniprot
Q8TAE6 (PPP1R14C) T73 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96C90 (PPP1R14B) T57 Uniprot

Research Backgrounds

Function:

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

PTMs:

Autophosphorylated on serine residues.

Subcellular Location:

Cell junction>Focal adhesion. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium. Cytoplasm>Myofibril>Sarcomere.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Subunit Structure:

Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity.

Family&Domains:

A PH-like domain is involved in phosphatidylinositol phosphate binding.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). miR-758-3p Interferes with Neuronal Apoptosis in Cerebral Ischemia-Reperfusion by Inhibiting ILK. Molecular neurobiology, 2025 (PubMed: 39937418) [IF=4.6]

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