CAPN1 Antibody | Affinity Biosciences

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CAPN1 Antibody - Western blot analysis of Hela cell lysates, using CAPN1 Antibody.

CAPN1 Antibody - DF6306 at 1/100 staining Rat muscle tissue by IHC-P.

Product:	CAPN1 Antibody
Catalog:	DF6306
Description:	Rabbit polyclonal antibody to CAPN1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	82kDa; 82kD(Calculated).
Uniprot:	P07384
RRID:	AB_2838272

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CAPN1 Antibody detects endogenous levels of total CAPN1.

RRID:

AB_2838272
Cite Format: Affinity Biosciences Cat# DF6306, RRID:AB_2838272.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ca2 activated neutral protease; Calcium activated neutral proteinase; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium dependent protease small subunit; Calcium-activated neutral proteinase 1; Calpain 1; Calpain 1 large subunit; Calpain 1, (mu/I) large subunit; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; CAN1_HUMAN; CANP 1; CANP; CANP small subunit; CANP1; CANPL 1; CANPL1; CAPN 1; CAPN1; Cell proliferation inducing protein 30; Cell proliferation-inducing gene 30 protein; Micromolar Calpain; Micromolar-calpain; Mu Calpain; muCANP; muCL;

Immunogens

Immunogen:

Uniprot:

P07384(CAN1_HUMAN) >>Visit HPA database.

Gene(ID):

CAPN1(823)

Expression:

P07384 CAN1_HUMAN:

Ubiquitous.

Description:

Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).

Sequence:

P07384 CAN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07384 As Substrate

P07384

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
K20	Ubiquitination	Uniprot
K36	Ubiquitination	Uniprot
Y42	Phosphorylation	Uniprot
K71	Ubiquitination	Uniprot
S77	Phosphorylation	Uniprot
S78	Phosphorylation	Uniprot
K79	Ubiquitination	Uniprot
T80	Phosphorylation	Uniprot
Y81	Phosphorylation	Uniprot
K84	Acetylation	Uniprot
K84	Ubiquitination	Uniprot
K86	Ubiquitination	Uniprot
S93	Phosphorylation	Uniprot
K193	Ubiquitination	Uniprot
K229	Methylation	Uniprot
K229	Ubiquitination	Uniprot
S232	Phosphorylation	Uniprot
Y235	Phosphorylation	Uniprot
K240	Ubiquitination	Uniprot
S251	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
K270	Ubiquitination	Uniprot
K280	Ubiquitination	Uniprot
S290	Phosphorylation	Uniprot
K328	Ubiquitination	Uniprot
T354	Phosphorylation	Uniprot
K359	Ubiquitination	Uniprot
S360	Phosphorylation	Uniprot
T362	Phosphorylation	Uniprot
K365	Ubiquitination	Uniprot
Y371	Phosphorylation	Uniprot
T374	Phosphorylation	Uniprot
S379	Phosphorylation	Uniprot
T380	Phosphorylation	Uniprot
Y387	Phosphorylation	Uniprot
K398	Ubiquitination	Uniprot
T404	Phosphorylation	Uniprot
Y410	Phosphorylation	Uniprot
S415	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
Y446	Phosphorylation	Uniprot
K461	Ubiquitination	Uniprot
S470	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
Y494	Phosphorylation	Uniprot
K505	Ubiquitination	Uniprot
S539	Phosphorylation	Uniprot
K564	Ubiquitination	Uniprot
K583	Ubiquitination	Uniprot
S622	Phosphorylation	Uniprot
S636	Phosphorylation	Uniprot

Research Backgrounds

P07384_CAN1_HUMAN

Function:

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

PTMs:

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Forms a heterodimer with a small (regulatory) subunit CAPNS1.

Family&Domains:

Belongs to the peptidase C2 family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

References

1). Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling. Biological trace element research, 2020 (PubMed: 31797207) [IF=3.4]

Restrictive clause

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CAPN1 Antibody - #DF6306