GRK2 Antibody - #BF0499
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# BF0499, RRID:AB_2833786.
Fold/Unfold
ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2;
Immunogens
Purified recombinant fragment of human GRK2 expressed in E. Coli.
- P25098 ARBK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL
PTMs - P25098 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y13 | Phosphorylation | P00533 (EGFR) , P12931 (SRC) | Uniprot |
| S29 | Phosphorylation | P17252 (PRKCA) , P05129 (PRKCG) , Q05655 (PRKCD) | Uniprot |
| K31 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| R50 | Methylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | P00533 (EGFR) , P12931 (SRC) | Uniprot |
| Y92 | Phosphorylation | P12931 (SRC) , P00533 (EGFR) | Uniprot |
| K94 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| Y356 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| K644 | Acetylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S670 | Phosphorylation | P27361 (MAPK3) , P24941 (CDK2) | Uniprot |
| S676 | Phosphorylation | Uniprot | |
| K677 | Ubiquitination | Uniprot | |
| R683 | Methylation | Uniprot | |
| S685 | Phosphorylation | P17612 (PRKACA) | Uniprot |
PTMs - P25098 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P05387 (RPLP2) | S102 | Uniprot |
| P05387 (RPLP2) | S105 | Uniprot |
| P07550 (ADRB2) | S355 | Uniprot |
| P07550 (ADRB2) | S356 | Uniprot |
| P07550 (ADRB2) | T384 | Uniprot |
| P07550 (ADRB2) | S396 | Uniprot |
| P07550 (ADRB2) | S401 | Uniprot |
| P07550 (ADRB2) | S407 | Uniprot |
| P07550 (ADRB2) | S411 | Uniprot |
| P08069 (IGF1R) | S1278 | Uniprot |
| P08172 (CHRM2) | S232 | Uniprot |
| P08172 (CHRM2) | S234 | Uniprot |
| P08172 (CHRM2) | S250 | Uniprot |
| P08172 (CHRM2) | S251 | Uniprot |
| P08172 (CHRM2) | T271 | Uniprot |
| P08172 (CHRM2) | S282 | Uniprot |
| P08172 (CHRM2) | S283 | Uniprot |
| P08172 (CHRM2) | S286 | Uniprot |
| P08172 (CHRM2) | T287 | Uniprot |
| P08172 (CHRM2) | S288 | Uniprot |
| P08172 (CHRM2) | S290 | Uniprot |
| P08172 (CHRM2) | S294 | Uniprot |
| P08172 (CHRM2) | T302 | Uniprot |
| P08172 (CHRM2) | T307 | Uniprot |
| P08172 (CHRM2) | S309 | Uniprot |
| P08172 (CHRM2) | T310 | Uniprot |
| P08172 (CHRM2) | S311 | Uniprot |
| P09497 (CLTB) | S205 | Uniprot |
| P15311 (EZR) | T567 | Uniprot |
| P18545 (PDE6G) | T62 | Uniprot |
| P21731-2 (TBXA2R) | S239 | Uniprot |
| P21731-2 (TBXA2R) | S357 | Uniprot |
| P26038 (MSN) | T558 | Uniprot |
| P30411 (BDKRB2) | S366 | Uniprot |
| P30411 (BDKRB2) | S373 | Uniprot |
| P30411 (BDKRB2) | S375 | Uniprot |
| P32245 (MC4R) | T312 | Uniprot |
| P32245 (MC4R) | S329 | Uniprot |
| P32245 (MC4R) | S330 | Uniprot |
| P35241 (RDX) | T564 | Uniprot |
| P35367 (HRH1) | S398 | Uniprot |
| P35372 (OPRM1) | T356 | Uniprot |
| P35372 (OPRM1) | S357 | Uniprot |
| P35372 (OPRM1) | S358 | Uniprot |
| P35372 (OPRM1) | S377 | Uniprot |
| P37840 (SNCA) | S129 | Uniprot |
| P41143 (OPRD1) | T358 | Uniprot |
| P41143 (OPRD1) | S363 | Uniprot |
| Q14940 (SLC9A5) | S702 | Uniprot |
| Q14940 (SLC9A5) | S709 | Uniprot |
| Q14940 (SLC9A5) | S711 | Uniprot |
| Q14940 (SLC9A5) | S712 | Uniprot |
| Q14940 (SLC9A5) | T714 | Uniprot |
| Q15796 (SMAD2) | T197 | Uniprot |
| Q16539 (MAPK14) | T123 | Uniprot |
| Q96A54 (ADIPOR1) | S7 | Uniprot |
| Q96A54 (ADIPOR1) | T24 | Uniprot |
| Q96A54 (ADIPOR1) | T53 | Uniprot |
| Q9Y2W7 (KCNIP3) | S95 | Uniprot |
Research Backgrounds
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).
Cytoplasm. Cell membrane.
Expressed in peripheral blood leukocytes.
Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Interacts with ADRB2.
The PH domain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Sensory system > Olfactory transduction.
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