Ferritin Light Chain Antibody - #DF6604

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Product: Ferritin Light Chain Antibody
Catalog: DF6604
Description: Rabbit polyclonal antibody to Ferritin Light Chain
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 19kDa; 20kD(Calculated).
Uniprot: P02792
RRID: AB_2838566

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Ferritin Light Chain Antibody detects endogenous levels of total Ferritin Light Chain.
RRID:
AB_2838566
Cite Format: Affinity Biosciences Cat# DF6604, RRID:AB_2838566.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; ferritin light polypeptide like 3; FRIL_HUMAN; FTL; LFTD; NBIA 3; NBIA3;

Immunogens

Immunogen:

A synthesized peptide derived from human Ferritin Light Chain, corresponding to a region within C-terminal amino acids.

Uniprot:

P02792(FRIL_HUMAN) >>Visit HPA database.

Gene(ID):
FTL(2512)
Description:
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.
Sequence:
MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
80
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Family&Domains:

Belongs to the ferritin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1. Redox biology, 2024 (PubMed: 39326069) [IF=10.7]
2). Fine particulate matter potentiates Th17-cell pathogenicity in experimental autoimmune uveitis via ferroptosis. Ecotoxicology and environmental safety, 2024 (PubMed: 39232294) [IF=6.2]
3). Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development. Ecotoxicology and environmental safety, 2024 (PubMed: 38964057) [IF=6.2]
4). Zinc oxide nanoparticles induces cell death and consequently leading to incomplete neural tube closure through oxidative stress during embryogenesis. Cell biology and toxicology, 2024 (PubMed: 38958792) [IF=5.3]
5). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2024 (PubMed: 38189810) [IF=4.8]
6). Cigarette smoke extract induces ferroptosis in human retinal pigment epithelial cells. Heliyon, 2024 (PubMed: 39498038) [IF=4.0]
7). Piceatannol upregulates USP14-mediated GPX4 deubiquitination to inhibit neuronal ferroptosis caused by cerebral ischemia-reperfusion in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 2025 (PubMed: 39880152) [IF=3.9]
8). NCOA4‐mediated ferritinophagy promoted inflammatory responses in periodontitis. JOURNAL OF PERIODONTAL RESEARCH, 2021 (PubMed: 33533512) [IF=3.4]

Application: IHC    Species: Human    Sample: gingival tissues

FIGURE 1 Increased expression of iron metabolism-related genes in gingival tissues. Samples from normal control (NC) (n = 8) and chronic periodontitis patients (CP) (n = 12) were stained for NCOA4 (A&B), FTH1 (C&D), and FTL (E&F). The scale bar represents 100 µm. Figures (a1/2–f1/2) showed higher magnifications of the same sections. The scale bar represented 20 µm. (G) Semi-quantitative results (mean ±SD) of immunostaining for NCOA4, FTH1, and FTL in the normal control and periodontitis group. *p < .05; **p < .01
9). Dexmedetomidine Blocks NCOA4-Dependent Ferritinophagy to Confer Ferroptosis Resistance in Lung Ischemia Reperfusion Injury via Targeting NRF2. Journal of biochemical and molecular toxicology, 2025 (PubMed: 39887555) [IF=3.2]
10). Identification of hepatocellular carcinoma-related subtypes and development of a prognostic model: a study based on ferritinophagy-related genes. Discover oncology, 2023 (PubMed: 37555866) [IF=2.2]

Application: IHC    Species: human    Sample: tumor tissue

Fig. 16 A expression of FTH1 mRNA in different liver cancer cells and normal liver cells; B expression of FTL mRNA in different liver cancer cells and normal liver cells; C the HE staining results of the patient tissue samples. D expression of FTH1, FTL in tumor tissue; *P 
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