PDE1B Antibody - #DF6826

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Product: PDE1B Antibody
Catalog: DF6826
Description: Rabbit polyclonal antibody to PDE1B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 61kDa; 61kD(Calculated).
Uniprot: Q01064
RRID: AB_2838786

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
PDE1B Antibody detects endogenous levels of total PDE1B.
RRID:
AB_2838786
Cite Format: Affinity Biosciences Cat# DF6826, RRID:AB_2838786.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5''-cyclic nucleotide phosphodiesterase 1B; 63 kDa Cam PDE; 63 kDa Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B; Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calmodulin stimulated phosphodiesterase PDE1B1; Cam PDE 1B; Cam PDE1B; Cam-PDE 1B; PDE 1B; PDE1B; PDE1B_HUMAN; PDE1B1; PDES 1B; PDES1B; Phosphodiesterase 1B; Phosphodiesterase 1B calmodulin dependent; Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B;

Immunogens

Immunogen:
Uniprot:

Q01064(PDE1B_HUMAN) >>Visit HPA database.

Gene(ID):
PDE1B(5153)
Description:
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Sequence:
MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAVYIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Dog
92
Pig
83
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01064 As Substrate

Site PTM Type Enzyme
C17 S-Nitrosylation
Y41 Phosphorylation
Y62 Phosphorylation
Y71 Phosphorylation
T144 Phosphorylation
T152 Phosphorylation
T201 Phosphorylation
S363 Phosphorylation
S372 Phosphorylation
T375 Phosphorylation
S382 Phosphorylation
S443 Phosphorylation
S452 Phosphorylation
S454 Phosphorylation
S466 Phosphorylation

Research Backgrounds

Function:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Renin secretion.

References

1). Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis. Journal of Oncology, 2021 (PubMed: 33564309)

Application: IHC    Species: Human    Sample: tumor tissues

Figure 14 Immunohistochemical staining results.

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