Product: Rab10 Antibody
Catalog: BF0441
Description: Mouse monoclonal antibody to Rab10
Application: WB IF/ICC ELISA
Reactivity: Human, Mouse
Mol.Wt.: 22.5kDa; 23kD(Calculated).
Uniprot: P61026
RRID: AB_2833828

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Product Info

ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1804]
Rab10 antibody detects endogenous levels of total Rab10.
Cite Format: Affinity Biosciences Cat# BF0441, RRID:AB_2833828.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


GTP binding protein RAB10; Rab10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10;



Purified recombinant fragment of human Rab10 expressed in E. Coli.

P61026 RAB10_HUMAN:

Expressed in the hippocampus (PubMed:29562525). Expressed in neutrophils (at protein level) (PubMed:29127255).

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking.

PTMs - P61026 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
Y6 Phosphorylation
S18 Phosphorylation
T41 Phosphorylation
K49 Ubiquitination
T50 Phosphorylation
K56 Ubiquitination
T65 Phosphorylation
T73 Phosphorylation
T76 Phosphorylation
S77 Phosphorylation
K102 Acetylation
K102 Ubiquitination
K123 Ubiquitination
K136 Ubiquitination
R147 Methylation
K154 Ubiquitination
T165 Phosphorylation
T174 Phosphorylation
K177 Ubiquitination
S181 Phosphorylation
S187 Phosphorylation
S188 Phosphorylation
T193 Phosphorylation
K196 Acetylation

Research Backgrounds


The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Plays also a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis.


Phosphorylation of Thr-73 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

Subcellular Location:

Cytoplasmic vesicle membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane. Golgi apparatus>trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle>Phagosome membrane. Cytoplasm>Cytoskeleton>Cilium basal body. Endoplasmic reticulum membrane. Cytoplasm>Perinuclear region.
Note: Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium when phosphorylated by LRRK2 on Thr-73 (PubMed:20576682, PubMed:30398148). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the hippocampus. Expressed in neutrophils (at protein level).

Subunit Structure:

Interacts with MYO5A; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles. Interacts with GDI1 and with GDI2; negatively regulates RAB10 association with membranes and activation. Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1 (By similarity). Interacts with EXOC4; probably associates with the exocyst (By similarity). Interacts (GTP-bound form) with MICALCL, MICAL1, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1 two molecules of RAB10 can bind to one molecule of MICAL1. Interacts with TBC1D13 (By similarity). Interacts with SEC16A (By similarity). Interacts with CHM and CHML. Interacts with LRRK2; interaction facilitates phosphorylation of Thr-73. Interacts (when phosphorylated on Thr-73) with RILPL1 and RILPL2.


Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

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