Product: GRM1 Antibody
Catalog: DF7120
Description: Rabbit polyclonal antibody to GRM1
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 132kDa; 132kD(Calculated).
Uniprot: Q13255
RRID: AB_2839074

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
GRM1 Antibody detects endogenous levels of total GRM1.
RRID:
AB_2839074
Cite Format: Affinity Biosciences Cat# DF7120, RRID:AB_2839074.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Glutamate receptor metabotropic 1; glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1-Alpha; GRM1_HUMAN; GRM1A; Metabotropic glutamate receptor 1; mGlu1; mGluR1; MGLUR1-Alpha; MGLUR1A; SCAR13;

Immunogens

Immunogen:

A synthesized peptide derived from human GRM1, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q13255 GRM1_HUMAN:

Detected in brain.

Description:
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Sequence:
MVGLLLFFFPAIFLEVSLLPRSPGRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRCLPDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKRICTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEFTCKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKAGAGNANSNGKSVSWSEPGGGQVPKGQHMWHRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDTSTKTLYNVEEEEDAQPIRFSPPGSPSMVVHRRVPSAATTPPLPSHLTAEETPLFLAEPALPKGLPPPLQQQQQPPPQQKSLMDQLQGVVSNFSTAIPDFHAVLAGPGGPGNGLRSLYPPPPPPQHLQMLPLQLSTFGEELVSPPADDDDDSERFKLLQEYVYEHEREGNTEEDELEEEEEDLQAASKLTPDDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVSYASVILRDYKQSSSTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. May function in the light response in the retina (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain.

Family&Domains:

Belongs to the G-protein coupled receptor 3 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). PLCβ4 driven by cadmium-exposure during gestation and lactation contributes to cognitive deficits by suppressing PIP2/PLCγ1/CREB/BDNF signaling pathway in male offspring. Journal of hazardous materials, 2024 (PubMed: 38820747) [IF=12.2]

Application: WB    Species: Rat    Sample: hippocampus

Fig. 6. PLCβ4 is activated by mGluR1 to suppress the PIP2/PLCγ1/CREB/BDNF signaling pathway in hippocampal neurons. A) Immunoblots and quantification of mGluR1, PLCβ4, p-CREB and BDNF in neurons after 2 μM CdCl2 (Cd2) exposure, n = 3. B-E) The expression of mGluR1 in the hippocampus of male offspring following maternal Cd exposure at PND 21 B), PND 35 C), PND 56 D), and in old age E) was assessed by western blotting, n = 4/6/7/8. F-J) After transfection with 60 nM si-PLCβ4 for 24 h, a final concentration of 10 μM mGluR1 agonist (Ro-01–6128) was administered to hippocampal neurons for 24 h. F-J) Immunoblots and quantitative analysis of PLCβ4 G), p-PLCγ1 H), p-CREB (Ser133) I), and BDNF J), n = 4. K) After knocking down PLCβ4, hippocampal neurons were treated with an mGluR1 agonist for 24 h, and the mRNA levels of BDNF-I, BDNF-IV, and BDNF-VI were assessed by qPCR, n = 3. L) The representative images of Tuj1+ hippocampal neurons (red) treated with Ro-01–6128 before the knock-down of PLCβ4 (Scale bar: 50 µm) and the quantification of average neurite length, n = 3. After transfection with 60 nM si-PLCβ4 for 24 h, mGluR1 agonist (Ro-01–6128, 10 μM) and CdCl2 (2 μM) were administered to hippocampal neurons for 24 h. M-Q) The representative immunoblots M) and quantification of PLCβ4 N), p-PLCγ1 O), p-CREB (Ser133) P), and BDNF Q), n = 3. R-S) The representative images of MAP2+ hippocampal neurons (Scale bar: 50 µm) and the quantification of average neurite length, n = 3. The hippocampal neurons were transfected with si-PLCβ4 for 24 h, followed by the treatment of TrkB inhibitor ANA-12 (10 nM) with or without CdCl2 (2 μM) for 24 h. T, W) The expression levels of PLCβ4 and p-TrKB (Tyr706) were detected by Western blotting, n = 3/4. U-V, X-Y) The representative images of MAP2+ hippocampal neurons (Scale bar: 50 µm) and the quantification of average neurite length, n = 3. Data are represented as the mean ± SEM. Statistical significance are calculated by one-way ANOVA with Tukey’s post-hoc test and Student’s t test. * P 

2). Peanut oil odor enhances the immunomodulatory effect on immunosuppressed mice by regulating the cAMP signaling pathway via the brain-spleen axis. Food & function, 2024 (PubMed: 38288526) [IF=5.1]

3). SIRT1 Activation Attenuates Bone Cancer Pain by Inhibiting mGluR1/5. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2019 (PubMed: 31270711) [IF=3.6]

Application: WB    Species: mouse    Sample: spinal cord

Fig. 4| Efect of intrathecal injections of SRT1720 on the levels of the mGluR1/5 protein and mRNA in the spinal cord of mice with BCP. a, b Western blots showing the levels of the mGluR1 protein. c, d Western blots showing the levels of the mGluR5 protein.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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