DUSP6 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of Hela whole cell lysates, using DUSP6 Antibody.

DF7228 at 1/100 staining Human gastric tissue by IHC-P.

DF7228 at 1/100 staining Human pancreatic cancer by IHC-P.

DF7228 staining Hela cells by IF/ICC.

Product:	DUSP6 Antibody
Catalog:	DF7228
Description:	Rabbit polyclonal antibody to DUSP6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	27kDa; 42kD(Calculated).
Uniprot:	Q16828
RRID:	AB_2839169

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

DUSP6 Antibody detects endogenous levels of total DUSP6.

RRID:

AB_2839169
Cite Format: Affinity Biosciences Cat# DF7228, RRID:AB_2839169.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dual specificity phosphatase 6; Dual specificity phosphatase 6 isoform a; Dual specificity protein phosphatase 6; Dual specificity protein phosphatase PYST1; DUS6_HUMAN; DUSP 6; DUSP 6a; Dusp6; DUSP6a; HH19; MAP kinase phosphatase 3; Mitogen activated protein kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP 3; MKP-3; MKP3; PYST 1; PYST1; Serine/threonine specific protein phosphatase;

Immunogens

Immunogen:

Uniprot:

Q16828(DUS6_HUMAN) >>Visit HPA database.

Gene(ID):

DUSP6(1848)

Expression:

Q16828 DUS6_HUMAN:

Expressed in keratinocytes (at protein level).

Description:

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Sequence:

Q16828 DUS6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16828 As Substrate

Q16828

Site	PTM Type	Enzyme	Source
K68	Ubiquitination		Uniprot
S159	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
S174	Phosphorylation	P27361 (MAPK3)	Uniprot
S178	Phosphorylation		Uniprot
S182	Phosphorylation		Uniprot
S197	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
K254	Ubiquitination		Uniprot
Y255	Phosphorylation		Uniprot
S300	Phosphorylation	P27361 (MAPK3)	Uniprot
K324	Ubiquitination		Uniprot
K327	Ubiquitination		Uniprot
S328	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot

Research Backgrounds

Q16828_DUS6_HUMAN

Function:

Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity). Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Expressed in keratinocytes (at protein level).

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

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DUSP6 Antibody - #DF7228