REV1 Antibody - #DF2581

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Product: REV1 Antibody
Catalog: DF2581
Description: Rabbit polyclonal antibody to REV1
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 138 kDa; 138kD(Calculated).
Uniprot: Q9UBZ9
RRID: AB_2839787

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
REV1 Antibody detects endogenous levels of total REV1.
RRID:
AB_2839787
Cite Format: Affinity Biosciences Cat# DF2581, RRID:AB_2839787.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIBP 80; AIBP80; Alpha integrin binding protein 80; Alpha integrin-binding protein 80; DNA repair protein REV 1; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV 1 (yeast homolog) like; REV 1; REV 1 homolog; REV 1 like; REV 1 protein; REV 1L; REV1 (yeast homolog) like; REV1; REV1 homolog (S. cerevisiae); REV1 homolog; REV1 like (yeast); REV1 like; Rev1 like terminal deoxycytidyl transferase; REV1 protein; Rev1-like terminal deoxycytidyl transferase; REV1_HUMAN; REV1L;

Immunogens

Immunogen:

A synthesized peptide derived from human REV1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UBZ9(REV1_HUMAN) >>Visit HPA database.

Gene(ID):
REV1(51455)
Expression:
Q9UBZ9 REV1_HUMAN:

Ubiquitous.

Description:
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Sequence:
MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGTSSTIFSGVAIYVNGYTDPSAEELRKLMMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSVQKGLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGRKQNGIPHPRGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTLQQLQQSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSKAAPSVPSKPSDCNFISNFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIMHVDMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKAADIPDSSLWENPDSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASYTHNIEAVSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQYHLKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKEFGPKTGQMLYRFCRGLDDRPVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAKFGGHGICDNIARTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSVQSSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESSGKWNGLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTGILPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRELKAAYDQRQRQGENSTHQQSASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEGPPAEKPLEELSASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQVVKYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
90
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

The C-terminal domain is necessary for protein interactions.

Belongs to the DNA polymerase type-Y family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

References

1). Elevated expression of REV1 is a predictor of unfavorable prognosis in patients with prostate cancer. , 2021

Application: WB    Species: Human    Sample: Prostate cancer tissues

Figure 1. Immunostaining of REV1 protein in prostate cancer, normal prostate, and hyperplasia tissues. (A) Immunostaining of TMA from 80 samples. (B) IRSs of REV1 protein in Prostate cancer, normal prostate, and hyperplasia tissues. Expression levels of REV1 protein in Prostate cancer tissues were significantly higher than that in non-cancerous prostate tissues (IRS: Prostate cancer = 4.08±1.30 vs. Normal = 2.50±0.71, P < 0.001; Prostate cancer = 4.08±1.30 vs. Hyperplasia = 3.15±1.14, P = 0.007). **mean P < 0.01; (C, D) Representative immunohistochemical photographs of REV1 protein in Prostate cancer tissues. (E, F) Representative immunohistochemical photographs of REV1 protein in normal and hyperplasia prostate tissues. The red arrows in (C, D) show positive staining cells.

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