5HT3A receptor Antibody - #DF2707

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Product: 5HT3A receptor Antibody
Catalog: DF2707
Description: Rabbit polyclonal antibody to 5HT3A receptor
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human
Mol.Wt.: 55 kDa,; 55kD(Calculated).
Uniprot: P46098
RRID: AB_2839913

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
5HT3A receptor Antibody detects endogenous levels of total 5HT3A receptor.
RRID:
AB_2839913
Cite Format: Affinity Biosciences Cat# DF2707, RRID:AB_2839913.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 HT 3; 5 HT3 receptor; 5 HT3A; 5 HT3A receptor; 5 HT3RA; 5 hydroxytryptamine (serotonin) receptor 3A; 5-HT-3; 5-HT3-A; 5-HT3A; 5-HT3R; 5-hydroxytryptamine receptor 3; 5-hydroxytryptamine receptor 3A; 5HT receptor; 5HT3 serotonin receptor; 5HT3A_HUMAN; 5HT3R; HTR3; Htr3a; Serotonin receptor 3A; Serotonin-gated ion channel receptor 3a; Serotonin-gated ion channel receptor;

Immunogens

Immunogen:

A synthesized peptide derived from human 5HT3A receptor, corresponding to a region within the internal amino acids.

Uniprot:

P46098(5HT3A_HUMAN) >>Visit HPA database.

Gene(ID):
HTR3A(3359)
Expression:
P46098 5HT3A_HUMAN:

Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.

Description:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.;
Sequence:
MLLWVQQALLALLLPTLLAQGEARRSRNTTRPALLRLSDYLLTNYRKGVRPVRDWRKPTTVSIDVIVYAILNVDEKNQVLTTYIWYRQYWTDEFLQWNPEDFDNITKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYIRHQGEVQNYKPLQVVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINISLWRLPEKVKSDRSVFMNQGEWELLGVLPYFREFSMESSNYYAEMKFYVVIRRRPLFYVVSLLLPSIFLMVMDIVGFYLPPNSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQDLQQPVPAWLRHLVLERIAWLLCLREQSTSQRPPATSQATKTDDCSAMGNHCSHMGGPQDFEKSPRDRCSPPPPPREASLAVCGLLQELSSIRQFLEKRDEIREVARDWLRVGSVLDKLLFHIYLLAVLAYSITLVMLWSIWQYA

Research Backgrounds

Function:

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

References

1). Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023 (PubMed: 36627132) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 2 5-HT mediated ferroptosis resistance is independent of canonical anti-ferroptosis pathway. A) Western blot analysis of 5-HT receptors in a panel of cancer cell lines. B) HT1080 cells expressing shRNA of 5-HT receptors were treated with RSL3 (200 nm) and 5-HT (10 µm). Cell viability was assessed 24 h thereafter using CCK8. C) Western blot analysis of arrestin β1 and arrestin β2 in 293T arrestin β1-/-, arrestin β2-/- DKO cells. D) Cell death measurement of 293T arrestin β1-/-, arrestin β2-/- DKO cells treated with RSL3 (2.5 µm) and Fer-1 (1 µm) for 8 h. E) Western blot analysis of p-mTOR and p-AMPK in a panel of cell lines upon 5-HT treatment (10 µm) for 12 h. F) RNA-seq analysis of HT1080 cells with or without 5-HT treatment (10 µm) for 12 h. G) Volcano plots showing the alteration of phospholipid components in HT1080 cells treated with 5-HT (10 µm) for 12 h.

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