Product: TWIST1 Antibody
Catalog: BF0018
Description: Mouse monoclonal antibody to TWIST1
Application: WB ELISA
Reactivity: Human
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: Q15672
RRID: AB_2834065

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Product Info

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB2043]
TWIST1 antibody detects endogenous levels of total TWIST1.
Cite Format: Affinity Biosciences Cat# BF0018, RRID:AB_2834065.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; TWIST; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; TWIST1; TWST1_HUMAN;



Purified recombinant fragment of human TWIST1 expressed in E. Coli.


Subset of mesodermal cells.

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1.

PTMs - Q15672 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation P28482 (MAPK1)
S18 Phosphorylation P68400 (CSNK2A1)
S20 Phosphorylation P68400 (CSNK2A1)
K38 Ubiquitination
S42 Phosphorylation P31749 (AKT1)
S45 Phosphorylation
S68 Phosphorylation Q15759 (MAPK11) , P45983 (MAPK8) , Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)
K73 Acetylation
S102 Phosphorylation
Y103 Phosphorylation
T121 Phosphorylation
S123 Phosphorylation P31749 (AKT1)
T137 Phosphorylation
K142 Ubiquitination
S144 Phosphorylation
K145 Ubiquitination

Research Backgrounds


Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Subset of mesodermal cells.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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