Product: Phospho-MAPKAPK5 (Thr182) Antibody
Catalog: AF0060
Description: Rabbit polyclonal antibody to Phospho-MAPKAPK5 (Thr182)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 60kDa; 54kD(Calculated).
Uniprot: Q8IW41
RRID: AB_2834103

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Phospho-MAPKAPK5 (Thr182) Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Threonine 182.
Cite Format: Affinity Biosciences Cat# AF0060, RRID:AB_2834103.
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5_HUMAN; MAPKAP kinase 5; MAPKAPK-5; MAPKAPK5; Mitogen activated protein kinase activated protein kinase 5; p38-regulated/activated protein kinase; PRAK;



Expressed ubiquitously.

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

PTMs - Q8IW41 As Substrate

Site PTM Type Enzyme
S93 Phosphorylation
S115 Phosphorylation P17612 (PRKACA)
K123 Ubiquitination
K166 Ubiquitination
T182 Phosphorylation P53778 (MAPK12) , P28482 (MAPK1) , O15264 (MAPK13) , P31152 (MAPK4) , P45984 (MAPK9) , Q15759 (MAPK11) , Q16539 (MAPK14) , Q16659 (MAPK6)
T186 Phosphorylation
Y188 Phosphorylation
K205 Ubiquitination
S206 Phosphorylation
S212 Phosphorylation
T214 Phosphorylation
Y242 Phosphorylation
K286 Ubiquitination
S348 Phosphorylation
S354 Phosphorylation
T368 Phosphorylation
S469 Phosphorylation
S472 Phosphorylation

PTMs - Q8IW41 As Enzyme

Substrate Site Source
O00418 (EEF2K) S377 Uniprot
O43524 (FOXO3) S215 Uniprot
O60229 (KALRN) S487 Uniprot
P04637 (TP53) S37 Uniprot
P04792 (HSPB1) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P07101-3 (TH) S19 Uniprot
P07101-3 (TH) S40 Uniprot
P07101-3 (TH) S404 Uniprot
P25685 (DNAJB1) S149 Uniprot
P25685 (DNAJB1) S151 Uniprot
P25685 (DNAJB1) S171 Uniprot
P31152 (MAPK4) S186 Uniprot
P47712 (PLA2G4A) S727 Uniprot
Q15382 (RHEB) S130 Uniprot
Q92599 (SEPT8) S163 Uniprot
Q92599 (SEPT8) S192 Uniprot

Research Backgrounds


Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.


Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously.

Subunit Structure:

Interacts with ERK3/MAPK6 and ERK4/MAPK4 (via FRIEDE motif); the interaction is direct (By similarity). Interacts with YWHAE; the interaction prevents phosphorylation of HSP27/HSPB1 leading to disrupt F-actin polymerization. Interacts with SQSTM1.


Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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