MMP3 Antibody | Affinity Biosciences

Product:	MMP3 Antibody
Catalog:	AF0217
Description:	Rabbit polyclonal antibody to MMP3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Rabbit, Dog
Mol.Wt.:	53kDa; 54kD(Calculated).
Uniprot:	P08254
RRID:	AB_2833347

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)

Clonality:

Polyclonal

Specificity:

MMP3 Antibody detects endogenous levels of total MMP3.

RRID:

AB_2833347
Cite Format: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

Immunogens

Immunogen:

Uniprot:

P08254(MMP3_HUMAN) >>Visit HPA database.

Gene(ID):

MMP3(4314)

Description:

MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.

Sequence:

P08254 MMP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

Rabbit

Sheep

Xenopus

Pig

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08254 As Substrate

P08254

Site	PTM Type	Source
Y18	Phosphorylation	Uniprot
Y116	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot

Research Backgrounds

P08254_MMP3_HUMAN

Function:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

References

1). Zou ZL et al. Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE 2021 Jul 12;91:153657. (PubMed: 34371251) [IF=7.9]

Application: WB Species: Rat Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC Species: Rat Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.

2). Pan Z et al. Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE 2022 Oct;105:154330. (PubMed: 35905566) [IF=7.9]

3). Feng K et al. Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy 2021 Jun;138:111531 (PubMed: 34311530) [IF=7.5]

4). Wang T et al. TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. INTERNATIONAL JOURNAL OF CANCER 2017 Oct 15;141(8):1630-1642 (PubMed: 28670703) [IF=6.4]

Application: IHC Species: human Sample: GCTB

Supplementary Figure 1 | The expression of MMP3 in GCT of bone. The expression level of MMP3 showed no significant correlation with TGF-β and PAR-1 expression.

5). Guo Q et al. PDLIM2 protects articular chondrocytes from lipopolysaccharide-induced apoptosis, degeneration and inflammatory injury through down-regulation of nuclear factor (NF)-κB signaling. International Immunopharmacology 2020 Aug 14;88:106883. (PubMed: 32805696) [IF=5.6]

Application: WB Species: Mouse Sample: chondrocytes

Fig. 2. Up-regulation of PDLIM2 attenuates LPS-induced injury in chondrocytes. Chondrocytes were transfected with empty vector (EV) or PDLIM2 expression vector for 48 h and then stimulated with 10 μg/ml of LPS for 6 h. (A) The relative mRNA expression of PDLIM2 was determined by RT-qPCR. (B, C) Protein expression of PDLIM2 was examined by Western blotting. (D) The effect of PDLIM2 overexpression on cell viability was detected by MTT assay. The effect of PDLIM2 overexpression on cell apoptosis was assessed by (E, F) TUNENL and (G, H) Annexin V-FITC/PI apoptosis assays. (I) The effect of PDLIM2 overexpression on MMP-3, MMP-13, COL2A1 and ACAN was evaluated by Western blotting. Quantitative analysis of protein expression of (J) MMP-3, (K) MMP-13, (L) COL2A1 and (M) ACAN. N = 3, **p < 0.01 and ***p < 0.001.

6). Jiang-Tao Wan et al. Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation. World Journal of Stem Cells (PubMed: 36483847) [IF=4.1]

7). Zheng L et al. Pathological changes and expression of lysine oxidases and matrix metalloproteinases‐1,‐2, and‐3 in ligaments of patients with haemophilic arthritis. HAEMOPHILIA 2022 Jan;28(1):145-150. (PubMed: 34697874) [IF=3.9]

Application: WB Species: human Sample: cruciate ligaments

Figure 5.|LOXs and MMP-1,-2,and -3protein expression levels in the cruciate ligaments of patients with OA or HA

8). Zhang S et al. Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. AUTOIMMUNITY 2021 Aug 25;1-12. (PubMed: 34431434) [IF=3.5]

9). Mao X et al. Inhibitors of PARP-1 exert inhibitory effects on the biological characteristics of hepatocellular carcinoma cells in vitro. Molecular Medicine Reports 2017 Jul;16(1):208-214 (PubMed: 28498459) [IF=3.4]

Application: WB Species: human Sample: HepG2

Figure 6. Effects of different concentrations of AG014699 and BSI‑201 on protein levels of PTEN, TIMP3 and MMP3 in HepG2 cells. (A) Blots showing protein expression following treatment with AG014699 and (B) quantification. (C) Blots showing protein expression following treatment with BSI‑201 and (D) quantification. * P<0.05, compared with the control group; ∆P<0.05, compared with the low dose group, ∆∆P<0.05, compared with the middle dose group. MMP, matrix metalloproteinase; TIMP 3, tissue inhibitor of metalloproteinase 3; PTEN, phosphatase and tensin homolog; CTRL, control.

10). Zhang K et al. Silencing of Vangl2 attenuates the inflammation promoted by Wnt5a via MAPK and NF-κB pathway in chondrocytes. Journal of Orthopaedic Surgery and Research 2021 Feb 15;16(1):136. (PubMed: 33588909) [IF=2.6]

Application: WB Species: Mice Sample: ATDC5 cells

Fig. 3 Knockdown of Vangl2 attenuated IL-1β-induced inflammatory activation in ATDC5. a, b The knockdown efficiency of siRNA targeting Vangl2 was detected by Western blotting (a) and RT-qPCR (b). c Effects of Vangl2 inhibition on MMP3, MMP9, MMP13, and IL-6 mRNA levels were measured by RT-qPCR. d Western blotting for MMP3, MMP9, MMP13, and IL-6 in three groups. Densitometric quantification values were normalized for GAPDH. **P < 0.01, ***P < 0.001

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MMP3 Antibody - #AF0217