Product: Cytochrome P450 2J2 Antibody
Catalog: AF0713
Description: Rabbit polyclonal antibody to Cytochrome P450 2J2
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 50kDa; 58kD(Calculated).
Uniprot: P51589
RRID: AB_2834224

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Cytochrome P450 2J2 Antibody detects endogenous levels of total Cytochrome P450 2J2.
Cite Format: Affinity Biosciences Cat# AF0713, RRID:AB_2834224.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Arachidonic acid epoxygenase; CP2J2_HUMAN; CPJ2; CYP2J2; CYPIIJ2; Cytochrome P450 2J2; Cytochrome P450 family 2 subfamily J polypeptide 2; Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; Cytochrome P450 subfamily IIJ polypeptide 2; Flavoprotein linked monooxygenase; Microsomal monooxygenase; OTTHUMP00000010550;


P51589 CP2J2_HUMAN:

Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue.

PTMs - P51589 As Substrate

Site PTM Type Enzyme
T20 Phosphorylation
T25 Phosphorylation
T141 Phosphorylation
T143 Phosphorylation
S259 Phosphorylation
T274 Phosphorylation
Y281 Phosphorylation
S490 Phosphorylation

Research Backgrounds


A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent. Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone. Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole. Catalyzes the sulfoxidation of fenbedazole.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).


Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

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